Promoter anchored interaction landscape of THP-1 macrophages captures early immune response processes.
Capture Hi-C
Chi-C
Genome wide association study (GWAS)
HiCap
Lipopolysaccharide (LPS)
Macrophages
THP-1
Journal
Cellular immunology
ISSN: 1090-2163
Titre abrégé: Cell Immunol
Pays: Netherlands
ID NLM: 1246405
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
05
02
2020
revised:
21
05
2020
accepted:
21
05
2020
pubmed:
28
6
2020
medline:
10
3
2021
entrez:
28
6
2020
Statut:
ppublish
Résumé
Macrophages are highly plastic immune cells with temporally distinct transcriptome changes upon lipopolysaccride (LPS) activation. However, to what extent transcriptome reprogramming is mediated via spatial chromatin looping is not well studied. We generated high resolution chromatin interaction maps for LPS-stimulated THP-1 macrophages (0 and 2 h) using capture Hi-C. Success of LPS stimulation was validated with transcriptome sequencing. Circa 2900 genes changed their interaction profile upon LPS stimulation and those gaining interactions were enriched for LPS response relevant processes, suggesting a substantial role for distal regulation. Immune and cardiovascular risk variants were enriched within the interacting regions, thereby providing insights into macrophage biology.
Identifiants
pubmed: 32592980
pii: S0008-8749(20)30308-7
doi: 10.1016/j.cellimm.2020.104148
pii:
doi:
Substances chimiques
Chromatin
0
Lipopolysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104148Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest There is no conflict of interest.