Targeted migration of pherophorin-S indicates extensive extracellular matrix dynamics in Volvox carteri.
Chlamydomonas reinhardtii
Volvox carteri
confocal laser scanning microscopy
extracellular matrix (ECM)
green algae
multicellularity
pherophorins
sex-inducer protein
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
06
02
2020
revised:
12
06
2020
accepted:
17
06
2020
pubmed:
1
7
2020
medline:
20
4
2021
entrez:
1
7
2020
Statut:
ppublish
Résumé
Hydroxyproline-rich glycoproteins (HRGPs) constitute a major group of proteins of the extracellular matrix (ECM). The multicellular green alga Volvox carteri is a suitable model organism in which to study the evolutionary transition to multicellularity, including the basic principles and characteristics of an ECM. In Volvox, the ECM is dominated by a single HRGP family: the pherophorins. Our inventory amounts to 117 pherophorin-related genes in V. carteri. We focused on a pherophorin with an unexpected characteristic: pherophorin-S is a soluble, non-cross-linked ECM protein. Using transformants expressing a YFP-tagged pherophorin-S we observed the synthesis and secretion of pherophorin-S by somatic cells in vivo, and we then traced the protein during its conspicuous migration to the ECM around prehatching juveniles and its localized concentration there. Our results provide insights into how an ECM zone surrounding the progeny is remotely affected by distantly located parental somatic cells. In view of the properties and migration of pherophorin-S, we conclude that pherophorin-S is likely to act as an ECM plasticizer to allow for dynamic ECM remodeling.
Substances chimiques
Algal Proteins
0
Glycoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2301-2317Informations de copyright
© 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
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