Localization of Tobacco Yellow Dwarf Virus Replication Using the In Plant Activation (INPACT) Expression Platform.
In Plant Activation (INPACT)
geminivirus
phloem
replication
tobacco
β-glucuronidase (GUS)
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
26 06 2020
26 06 2020
Historique:
received:
18
05
2020
revised:
24
06
2020
accepted:
24
06
2020
entrez:
2
7
2020
pubmed:
2
7
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Geminiviruses and their diseases are a considerable economic threat to a vast number of crops worldwide. Investigating how and where these viruses replicate and accumulate in their hosts may lead to novel molecular resistance strategies. In this study, we used the Rep-inducible In Plant Activation (INPACT) expression platform, based on the genome of tobacco yellow dwarf virus (TYDV), to determine where this model mastrevirus replicates in its host tobacco. By developing an infectious clone of TYDV and optimizing its delivery by agroinfiltration, we first established an efficient artificial infection process. When delivered into transgenic tobacco plants containing a TYDV-based INPACT cassette encoding the β-glucuronidase (GUS) reporter, we showed the virus activates GUS expression. Histology revealed that reporter gene expression was limited to phloem-associated cell types suggesting TYDV replication has a restricted tissue tropism.
Identifiants
pubmed: 32604765
pii: v12060688
doi: 10.3390/v12060688
pmc: PMC7354463
pii:
doi:
Substances chimiques
Glucuronidase
EC 3.2.1.31
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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