The effect of terminal globular domains on the response of recombinant mini-spidroins to fiber spinning triggers.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 06 2020
30 06 2020
Historique:
received:
04
02
2020
accepted:
11
06
2020
entrez:
2
7
2020
pubmed:
2
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Spider silk spidroins consist of long repetitive protein strands, flanked by globular terminal domains. The globular domains are often omitted in recombinant spidroins, but are thought to be essential for the spiders' natural spinning process. Mimicking this spinning process could be an essential step towards producing strong synthetic spider silk. Here we describe the production of a range of mini-spidroins with both terminal domains, and characterize their response to a number of biomimetic spinning triggers. Our results suggest that mini-spidroins which are able to form protein micelles due to the addition of both terminal domains exhibit shear-thinning, a property which native spidroins also show. Furthermore, our data also suggest that a pH drop alone is insufficient to trigger assembly in a wet-spinning process, and must be combined with salting-out for effective fiber formation. With these insights, we applied these assembly triggers for relatively biomimetic wet spinning. This work adds to the foundation of literature for developing improved biomimetic spinning techniques, which ought to result in synthetic silk that more closely approximates the unique properties of native spider silk.
Identifiants
pubmed: 32606438
doi: 10.1038/s41598-020-67703-1
pii: 10.1038/s41598-020-67703-1
pmc: PMC7327021
doi:
Substances chimiques
Dietary Fiber
0
Recombinant Proteins
0
Silk
0
Fibroins
9007-76-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10671Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M017702/1
Pays : United Kingdom
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