A functionally neutral single chain antibody to measure beta-1 integrin uptake and recycling.
endocytosis
integrin
recycling
scFv
trafficking
Journal
Traffic (Copenhagen, Denmark)
ISSN: 1600-0854
Titre abrégé: Traffic
Pays: England
ID NLM: 100939340
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
23
04
2020
revised:
27
06
2020
accepted:
28
06
2020
pubmed:
3
7
2020
medline:
28
4
2021
entrez:
3
7
2020
Statut:
ppublish
Résumé
Integrin-mediated cell adhesion and signaling are critical for many physiological processes. The dynamic turnover of integrins and their associated adhesion complexes through endocytic and recycling pathways has emerged as an important mechanism for controlling cell migration and invasion in cancer. Thus, the regulation of integrin trafficking and how this may be altered by disease-specific molecular mechanisms has generated considerable interest. However, current tools available to study integrin trafficking may cause artifacts and/or do not provide adequate kinetic information. Here, we report the generation of a functionally neutral and monovalent single chain antibody to quantitatively and qualitatively measure β1 integrin trafficking in cells. Our novel probe can be used in a variety of assays and allows for the biochemical characterization of rapid recycling of endogenous integrins. We also demonstrate its potential utility in live cell imaging, providing proof of principle to guide future integrin probe design.
Identifiants
pubmed: 32613646
doi: 10.1111/tra.12754
pmc: PMC7442622
mid: NIHMS1613815
doi:
Substances chimiques
Integrin beta1
0
Integrins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
590-602Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM042455
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM073165
Pays : United States
Organisme : NIGMS NIH HHS
ID : GM73165
Pays : United States
Organisme : NIGMS NIH HHS
ID : GM42455
Pays : United States
Informations de copyright
© 2020 The Authors. Traffic published by John Wiley & Sons Ltd.
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