In vitro antioxidant activity of Ficus carica L. latex from 18 different cultivars.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 07 2020
02 07 2020
Historique:
received:
02
02
2020
accepted:
08
06
2020
entrez:
4
7
2020
pubmed:
4
7
2020
medline:
16
12
2020
Statut:
epublish
Résumé
As synthetic antioxidants that are widely used in foods are known to cause detrimental health effects, studies on natural additives as potential antioxidants are becoming increasingly important. In this work, the total phenolic content (TPC) and antioxidant activity of Ficus carica Linn latex from 18 cultivars were investigated. The TPC of latex was calculated using the Folin-Ciocalteu assay. 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric ion reducing antioxidant power (FRAP) were used for antioxidant activity assessment. The bioactive compounds from F. carica latex were extracted via maceration and ultrasound-assisted extraction (UAE) with 75% ethanol as solvent. Under the same extraction conditions, the latex of cultivar 'White Genoa' showed the highest antioxidant activity of 65.91% ± 1.73% and 61.07% ± 1.65% in DPPH, 98.96% ± 1.06% and 83.04% ± 2.16% in ABTS, and 27.08 ± 0.34 and 24.94 ± 0.84 mg TE/g latex in FRAP assay via maceration and UAE, respectively. The TPC of 'White Genoa' was 315.26 ± 6.14 and 298.52 ± 9.20 µg GAE/mL via the two extraction methods, respectively. The overall results of this work showed that F. carica latex is a potential natural source of antioxidants. This finding is useful for further advancements in the fields of food supplements, food additives and drug synthesis in the future.
Identifiants
pubmed: 32616768
doi: 10.1038/s41598-020-67765-1
pii: 10.1038/s41598-020-67765-1
pmc: PMC7331616
doi:
Substances chimiques
Antioxidants
0
Flavonoids
0
Latex
0
Plant Extracts
0
Polyphenols
0
Solvents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10852Références
Li, A. N. et al. Resources and biological activities of natural polyphenols. Nutrients 6, 6020–6047. https://doi.org/10.3390/nu6126020 (2014).
doi: 10.3390/nu6126020
pubmed: 25533011
pmcid: 4277013
Murti, K. & Kumar, U. Antimicrobial activity of Ficus benghalensis and Ficus racemosa roots L. Am. J. Microbiol. 2, 21–24. https://doi.org/10.3844/ajmsp.2011.21.24 (2011).
doi: 10.3844/ajmsp.2011.21.24
Roberts, E. et al. Penicillin B, an antibacterial substance from Penicillium notatum. J. Biol. Chem. 147, 47–58 (1943).
Dueñas, M., Pérez-Alonso, J. J., Santos-Buelga, C. & Escribano-Bailón, T. Anthocyanin composition in fig (Ficus carica L.). J. Food Compos. Anal. 21, 107–115. https://doi.org/10.1016/j.jfca.2007.09.002 (2008).
doi: 10.1016/j.jfca.2007.09.002
Kolesnik, A., Kakhniashvili, T., Zherebin, Y. L., Golubev, V. & Pilipenko, L. Lipids of the fruit of Ficus carica. Chem. Nat. Compd. 22, 394–397. https://doi.org/10.1007/BF00579808 (1986).
doi: 10.1007/BF00579808
Jeong, W. S. & Lachance, P. Phytosterols and fatty acids in fig (Ficus carica, var. mission) fruit and tree components. J. Food Sci. 66, 278–281. https://doi.org/10.1111/j.1365-2621.2001.tb11332.x (2001).
doi: 10.1111/j.1365-2621.2001.tb11332.x
Idrus, R.B.H. et al. Ficus carica and bone health: A systematic review. Sains Malays. 47(11), 2741–2755. https://doi.org/10.17576/jsm-2018-4711-17 (2018).
doi: 10.17576/jsm-2018-4711-17
Raskovic, B., Lazic, J. & Polovic, N. Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening. J. Sci. Food Agric. 96, 576–582. https://doi.org/10.1002/jsfa.7126 (2016).
doi: 10.1002/jsfa.7126
pubmed: 25664689
Decaro, N. et al. Genomic characterization of a circovirus associated with fatal hemorrhagic enteritis in dog, Italy. PLoS ONE 9, e105909. https://doi.org/10.1371/journal.pone.0105909 (2014).
doi: 10.1371/journal.pone.0105909
pubmed: 25147946
pmcid: 4141843
Lazreg Aref, H. et al. In vitro cytotoxic and antiviral activities of Ficus carica latex extracts. Nat. Prod. Res. 25, 310–319. https://doi.org/10.1080/14786419.2010.528758 (2011).
doi: 10.1080/14786419.2010.528758
pubmed: 21294043
Lazreg-Aref, H., Mars, M., Fekih, A., Aouni, M. & Said, K. Chemical composition and antibacterial activity of a hexane extract of Tunisian caprifig latex from the unripe fruit of Ficus carica. Pharm. Biol. 50, 407–412. https://doi.org/10.3109/13880209.2011.608192 (2012).
doi: 10.3109/13880209.2011.608192
pubmed: 22136172
Faleh, E. et al. Influence of Tunisian Ficus carica fruit variability in phenolic profiles and in vitro radical scavenging potential. Rev. Bras. Farmacogn. 22, 1282–1289. https://doi.org/10.1590/S0102-695X2012005000132 (2012).
doi: 10.1590/S0102-695X2012005000132
Oliveira, A. P. et al. Chemical assessment and in vitro antioxidant capacity of Ficus carica latex. J. Agric. Food Chem. 58, 3393–3398. https://doi.org/10.1021/jf9039759 (2010).
doi: 10.1021/jf9039759
pubmed: 20158255
Zaynoun, S. et al. Ficus carica; isolation and quantification of the photoactive components. Contact Derm. 11, 21–25. https://doi.org/10.1111/j.1600-0536.1984.tb00164.x (1984).
doi: 10.1111/j.1600-0536.1984.tb00164.x
pubmed: 6744838
Oliveira, A. P. et al. Further insight into the latex metabolite profile of Ficus carica. J. Agric. Food Chem. 58, 10855–10863. https://doi.org/10.1021/jf1031185 (2010).
doi: 10.1021/jf1031185
pubmed: 20923221
Chatha, S. A. S., Hussain, A. I., Bajwa, J. U. R. & Sagir, M. Antioxidant activity of different solvent extracts of rice bran at accelerated storage of sunflower oil. J. Food Lipids. 13, 424–433. https://doi.org/10.1111/j.1745-4522.2006.00068.x (2006).
doi: 10.1111/j.1745-4522.2006.00068.x
Bouras, M. et al. Optimization of microwave-assisted extraction of polyphenols from Quercus bark. Ind. Crop. Prod. 77, 590–601. https://doi.org/10.1016/j.indcrop.2015.09.018 (2015).
doi: 10.1016/j.indcrop.2015.09.018
Dahmoune, F., Nayak, B., Moussi, K., Remini, H. & Madani, K. Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves. Food Chem. 166, 585–595. https://doi.org/10.1016/j.foodchem.2014.06.066 (2015).
doi: 10.1016/j.foodchem.2014.06.066
pubmed: 25053097
Ranic, M. et al. Optimization of microwave-assisted extraction of natural antioxidants from spent espresso coffee grounds by response surface methodology. J. Clean Prod. 80, 69–79. https://doi.org/10.1016/j.jclepro.2014.05.060 (2014).
doi: 10.1016/j.jclepro.2014.05.060
Ghafoor, K., Park, J. & Choi, Y. H. Optimization of supercritical fluid extraction of bioactive compounds from grape (Vitis labrusca B.) peel by using response surface methodology. Innov. Food Sci. Emerg. Technol. 11, 485–490. https://doi.org/10.1016/j.ifset.2010.01.013 (2010).
doi: 10.1016/j.ifset.2010.01.013
Maran, J. P., Manikandan, S., Priya, B. & Gurumoorthi, P. Box-Behnken design based multi-response analysis and optimization of supercritical carbon dioxide extraction of bioactive flavonoid compounds from tea (Camellia sinensis L.) leaves. J. Food Sci. Technol. 52, 92–104. https://doi.org/10.1007/s13197-013-0985-z (2015).
doi: 10.1007/s13197-013-0985-z
Oluwatoyin, O., Akpevweoghene, R. & Emoghwa, M. Methanol extracts of Strophanthus hispidus exhibit anti-apoptotic effects via alteration of cytochrome c and caspase 3 levels in rats with myocardial infarction. Chem. Pap. 74(2), 521–528. https://doi.org/10.1007/s11696-019-00894-8 (2020).
doi: 10.1007/s11696-019-00894-8
Shahinuzzaman, M. et al. Optimization of extraction parameters for antioxidant and total phenolic content of Ficus carica L. Latex from white genoa cultivar. Asian J. Chem. 31, 1859–1865. https://doi.org/10.14233/ajchem.2019.21946 (2019).
doi: 10.14233/ajchem.2019.21946
Japón-Luján, R., Luque-Rodríguez, J. & De Castro, M. L. Dynamic ultrasound-assisted extraction of oleuropein and related biophenols from olive leaves. J. Chromatogr. A. 1108, 76–82. https://doi.org/10.1016/j.chroma.2005.12.106 (2006).
doi: 10.1016/j.chroma.2005.12.106
pubmed: 16442552
Toma, M., Vinatoru, M., Paniwnyk, L. & Mason, T. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. Ultrason. Sonochem. 8, 137–142. https://doi.org/10.1016/S1350-4177(00)00033-X (2001).
doi: 10.1016/S1350-4177(00)00033-X
pubmed: 11326609
Pietrzak, W., Nowak, R. & Olech, M. Effect of extraction method on phenolic content and antioxidant activity of mistletoe extracts from Viscum album subsp. abietis. Chem. Pap. 68(7), 976–982. https://doi.org/10.2478/s11696-013-0524-4 (2014).
doi: 10.2478/s11696-013-0524-4
Pan, Z., Qu, W., Ma, H., Atungulu, G. G. & McHugh, T. H. Continuous and pulsed ultrasound-assisted extractions of antioxidants from pomegranate peel. Ultrason. Sonochem. 18, 1249–1257. https://doi.org/10.1016/j.ultsonch.2011.05.015 (2011).
doi: 10.1016/j.ultsonch.2011.05.015
pubmed: 21317015
Vinatoru, M. An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason. Sonochem. 8, 303–313. https://doi.org/10.1016/S1350-4177(01)00071-2 (2001).
doi: 10.1016/S1350-4177(01)00071-2
pubmed: 11441615
Sithisarn, P., Supabphol, R. & Gritsanapan, W. Comparison of free radical scavenging activity of Siamese neem tree (Azadirachta indica A Juss. Var. siamensis Valeton) leaf extracts prepared by different methods of extraction. Med. Prin. Pract. 15, 219–222. https://doi.org/10.1159/000092185 (2006).
doi: 10.1159/000092185
Vongsak, B. et al. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind. Crop. Prod. 44, 566–571. https://doi.org/10.1016/j.indcrop.2012.09.021 (2013).
doi: 10.1016/j.indcrop.2012.09.021
Alam, M. N., Wahed, T. B., Sultana, F., Ahmed, J. & Hasan, M. In vitro antioxidant potential of the methanolic extract of Bacopa monnieri L. Turk. J. Pharm. Sci. 9(3), 285–292 (2012).
Pisoschi, A. M. & Negulescu, G. P. Methods for total antioxidant activity determination: a review. Biochem. Anal. Biochem. 1, 1–10. https://doi.org/10.4172/2161-1009.1000106 (2011).
doi: 10.4172/2161-1009.1000106
Wang, J. et al. Cytotoxicity of fig fruit latex against human cancer cells. Food Chem. Toxic. 46, 1025–1033. https://doi.org/10.1016/j.fct.2007.10.042 (2008).
doi: 10.1016/j.fct.2007.10.042
Yen, G. C. & Duh, P. D. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active-oxygen species. J. Agric Food Chem. 42, 629–632. https://doi.org/10.1021/jf00039a005 (1994).
doi: 10.1021/jf00039a005
Gorinstein, S. et al. A comparative study of phenolic compounds and antioxidant and antiproliferative activities in frequently consumed raw vegetables. Eur. Food Res. Technol. 228, 903–911. https://doi.org/10.1089/jmf.2009.0018 (2009).
doi: 10.1089/jmf.2009.0018
Teixeira, D. M., Canelas, V. C., do Canto, A. M., Teixeira, J. & Dias, C. B. HPLC-DAD quantification of phenolic compounds contributing to the antioxidant activity of Maclura pomifera, Ficus carica and Ficus elastica extracts. Anal. Lett. 42, 2986–3003. https://doi.org/10.1080/00032710903276646 (2009).
doi: 10.1080/00032710903276646
Oyaizu, M. Studies on products of browning reaction. Jpn. J. Nutr. Diet. 44, 307–315. https://doi.org/10.5264/eiyogakuzashi.44.307 (1986).
doi: 10.5264/eiyogakuzashi.44.307
Batterman, S. A. et al. Breath, urine, and blood measurements as biological exposure indices of short-term inhalation exposure to methanol. Int. Arch. Occup. Environ. Health. 71, 325–335. https://doi.org/10.1007/s004200050288 (1998).
doi: 10.1007/s004200050288
pubmed: 9749971
Bouchard, M., Brunet, R. C., Droz, P.-O. & Carrier, G. A biologically based dynamic model for predicting the disposition of methanol and its metabolites in animals and humans. Toxicol. Sci. 64, 169–184. https://doi.org/10.1093/toxsci/64.2.169 (2001).
doi: 10.1093/toxsci/64.2.169
pubmed: 11719699
Chuwers, P. et al. Neurobehavioral effects of low-level methanol vapor exposure in healthy human volunteers. Environ. Res. 71, 141–150. https://doi.org/10.1006/enrs.1995.1076 (1995).
doi: 10.1006/enrs.1995.1076
pubmed: 8977623
Mousavi, S. R., Namaei-Ghassemi, M., Layegh, M. & Afzal Aghaee, M. Determination of methanol concentrations in traditional herbal waters of different brands in Iran. Iran. J. Basic Med. Sci. 14(4), 361–368 (2011).
pubmed: 23493100
pmcid: 3586831
Akhtar, P., Yaakob, Z., Ahmed, Y. & Shahinuzzaman, M. Ficus species good sources of natural antioxidant drugs. Turk J. Pharm. Sci. https://doi.org/10.4274/tjps.67699 (2019).
doi: 10.4274/tjps.67699
Zhou, K. & Yu, L. Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT-Food Sci. Technol. 37, 717–721. https://doi.org/10.1016/j.lwt.2004.02.008 (2004).
doi: 10.1016/j.lwt.2004.02.008
Arya, V., Thakur, N. & Kashyap, C. Preliminary phytochemical analysis of the extracts of Psidium leaves. J. Pharma. Phytochem. 1, 1–5. https://doi.org/10.5829/idosi.mejsr.2014.19.11.11415 (2012).
doi: 10.5829/idosi.mejsr.2014.19.11.11415
Al-Mansoub, M. A., Asmawi, M. Z. & Murugaiyah, V. Effect of extraction solvents and plant parts used on the antihyperlipidemic and antioxidant effects of Garcinia atroviridis: a comparative study. J. Sci. Food Agric. 94, 1552–1558. https://doi.org/10.1002/jsfa.6456 (2014).
doi: 10.1002/jsfa.6456
pubmed: 24166055
Tan, P., Tan, C. & Ho, C. Antioxidant properties: effects of solid-to-solvent ratio on antioxidant compounds and capacities of Pegaga (Centella asiatica). Int. Food Res. J. 18(2), 557–562 (2011).
Zhang, S. Q., Bi, H. M. & Liu, C. J. Extraction of bio-active components from Rhodiola sachalinensis under ultrahigh hydrostatic pressure. Sep. Purif. Technol. 57, 277–282. https://doi.org/10.1016/j.seppur.2007.04.022 (2007).
doi: 10.1016/j.seppur.2007.04.022
Sulaiman, S. F., Sajak, A. A. B., Ooi, K. L. & Seow, E. M. Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables. J. Food Compos. Anal. 24, 506–515. https://doi.org/10.1016/j.jfca.2011.01.020 (2011).
doi: 10.1016/j.jfca.2011.01.020
Azwanida, N. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants. 4, 3–8. https://doi.org/10.4172/2167-0412.1000196 (2015).
doi: 10.4172/2167-0412.1000196
Paech, K., Tracey, M. V., Linskens, H. F. & Sanwal, B. D. Moderne Methoden der Pflanzenanalyse/Modern Methods of Plant Analysis 1st edn, 26–55 (Springer, New York, 2013).
Bruno, B. J., Miller, G. D. & Lim, C. S. Basics and recent advances in peptide and protein drug delivery. Ther. Deliv. 4(11), 1443–1467. https://doi.org/10.4155/tde.13.104 (2013).
doi: 10.4155/tde.13.104
pubmed: 24228993
pmcid: 3956587
Bajracharya, R., Song, J. G., Back, S. Y. & Han, H. K. Recent advancements in non-invasive formulations for protein drug delivery. Comput. Struct. Biotechnol. J. 17, 1290–1308. https://doi.org/10.1016/j.csbj.2019.09.004 (2019).
doi: 10.1016/j.csbj.2019.09.004
pubmed: 31921395
pmcid: 6944732
Mnari, A. B., Harzallah, A., Amri, Z., Dhaou Aguir, S. & Hammami, M. Phytochemical content, antioxidant properties, and phenolic profile of tunisian raisin varieties (Vitis vinifera L.). Int J. Food Prop. 19, 578–590. https://doi.org/10.1080/10942912.2015.1038720 (2016).
doi: 10.1080/10942912.2015.1038720
Harzallah, A., Bhouri, A. M., Amri, Z., Soltana, H. & Hammami, M. Phytochemical content and antioxidant activity of different fruit parts juices of three figs (Ficus carica L.) varieties grown in Tunisia. Ind. Crop. Product. 83, 255–267. https://doi.org/10.1016/j.indcrop.2015.12.043 (2016).
doi: 10.1016/j.indcrop.2015.12.043
Huang, D., Ou, B. & Prior, R. L. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53, 1841–1856. https://doi.org/10.1021/jf030723c (2005).
doi: 10.1021/jf030723c
pubmed: 15769103
Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L. & Byrne, D. H. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 19, 669–675. https://doi.org/10.1016/j.jfca.2006.01.003 (2006).
doi: 10.1016/j.jfca.2006.01.003
Connor, A. M., Luby, J. J., Hancock, J. F., Berkheimer, S. & Hanson, E. J. Changes in fruit antioxidant activity among blueberry cultivars during cold-temperature storage. J. Agric. Food Chem. 50, 893–898. https://doi.org/10.1021/jf011212y (2002).
doi: 10.1021/jf011212y
pubmed: 11829664
Ajmal, M. et al. Exploring the nutritional characteristics of different parts of fig in relation to hypoglycemic potential. Pak. J. Life Soc. Sci. 14(2), 115–122 (2016).
Belguith-Hadriche, O. et al. HPLC-DAD-QTOF-MS profiling of phenolics from leaf extracts of two Tunisian fig cultivars: potential as a functional food. Biomed. Pharm. 89, 185–193. https://doi.org/10.1016/j.biopha.2017.02.004 (2017).
doi: 10.1016/j.biopha.2017.02.004
Debib, A., Tir-Touil, A., Mothana, R., Meddah, B. & Sonnet, P. Phenolic content, antioxidant and antimicrobial activities of two fruit varieties of Algerian Ficus carica L. J. Food Biochem. 38, 207–215. https://doi.org/10.1111/jfbc.12039 (2014).
doi: 10.1111/jfbc.12039
Ghazi, F., Rahmat, A., Yassin, Z., Ramli, N. S. & Buslima, N. A. Determination of total polyphenols and nutritional composition of two different types of Ficus carica leaves cultivated in Saudi Arabia. Pak. J. Nutr. 11, 1061–1065. https://doi.org/10.3923/pjn.2012.1061.1065 (2012).
doi: 10.3923/pjn.2012.1061.1065
Hoxha, L., Kongoli, R. & Hoxha, M. Antioxidant activity of some dried autochthonous Albanian fig (Ficus carica) cultivars. Int J. Crop. Sci. Technol. 1(2), 20–26 (2015).
Jokić, S. et al. Influence of extraction type on the total phenolics, total flavonoids and total colour change of different varieties of fig extracts. Hrana Zdravlju Bolesti 3, 90–95 (2014).
Mahmoudi, S., Khali, M., Benkhaled, A., Benamirouche, K. & Baiti, I. Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian Ficus carica L. varieties. Asian Pac. J. Trop. Biomed. 6, 239–245. https://doi.org/10.1016/j.apjtb.2015.12.010 (2016).
doi: 10.1016/j.apjtb.2015.12.010
Abu Bakar, U., et al. Sperm Proteomics analysis of diabetic induced male rats as influenced by Ficus carica leaf extract. Processes. 8(4), 395. https://doi.org/10.3390/pr8040395 (2020).
doi: 10.3390/pr8040395
Asamenew, G., Bisrat, D., Mazumder, A. & Asres, K. In vitro antimicrobial and antioxidant activities of anthrone and chromone from the latex of Aloe harlana Reynolds. Phytother. Res. 25, 1756–1760. https://doi.org/10.1002/ptr.3482 (2011).
doi: 10.1002/ptr.3482
pubmed: 21452374
De Marino, S. et al. Identification of minor secondary metabolites from the latex of Croton lechleri (Muell-Arg) and evaluation of their antioxidant activity. Molecules 13, 1219–1229. https://doi.org/10.3390/molecules13061219 (2008).
doi: 10.3390/molecules13061219
pubmed: 18596648
pmcid: 6245398
Hernandez-Hernandez, A. et al. Antimicrobial and anti-inflammatory activities, wound-healing effectiveness and chemical characterization of the latex of Jatropha neopauciflora Pax. J. Ethnopharm. 204, 1–7. https://doi.org/10.1016/j.jep.2017.04.003 (2017).
doi: 10.1016/j.jep.2017.04.003
Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. & Shahidi, F. Gelatin hydrolysate from blacktip shark skin prepared using papaya latex enzyme: antioxidant activity and its potential in model systems. Food Chem. 135, 1118–1126. https://doi.org/10.1016/j.foodchem.2012.05.080 (2012).
doi: 10.1016/j.foodchem.2012.05.080
pubmed: 22953833
Murthy, H. N., Joseph, K. S., Payamalle, S., Dalawai, D. & Ganapumane, V. Chemical composition, larvicidal and antioxidant activities of latex from Garcinia morella (Gaertn.) Desr. J. Parasit. Dis. 41, 666–670. https://doi.org/10.1007/s12639-016-0863-5 (2017).
doi: 10.1007/s12639-016-0863-5
pubmed: 28848256
Oskoueian, E. et al. Antioxidant, anti-inflammatory and anticancer activities of methanolic extracts from Jatropha curcas Linn. J. Med. Plants Res. 5, 49–57 (2011).
Sayed, A.E.-D.H., Mohamed, N. H., Ismail, M. A., Abdel-Mageed, W. M. & Shoreit, A. A. Antioxidant and antiapoptotic activities of Calotropis procera latex on Catfish (Clarias gariepinus) exposed to toxic 4-nonylphenol. Ecotoxicol. Environ. Saf. 128, 189–194. https://doi.org/10.1016/j.ecoenv.2016.02.023 (2016).
doi: 10.1016/j.ecoenv.2016.02.023
Siriwong, S., Rungvichaniwat, A., Klinpituksa, P., Musa, K. H. & Abdullah, A. Quantitative analysis of total phenolic contents and antioxidant activity of fresh field natural rubber latex. Macromol. Sympos. 1, 265–272. https://doi.org/10.1002/masy.201400101 (2015).
doi: 10.1002/masy.201400101