Contributions to Arabinogalactan Protein Analysis.
Arabidopsis
/ chemistry
Cell Wall
/ chemistry
Chromatography, Gel
/ methods
Electrophoresis
/ methods
Glucosides
/ chemistry
Immunohistochemistry
/ methods
Mucoproteins
/ analysis
Pisum sativum
/ chemistry
Phloroglucinol
/ analogs & derivatives
Plant Mucilage
/ analysis
Plant Proteins
/ analysis
Plant Roots
/ chemistry
Arabinogalactan proteins
Cell wall
Electrophoresis
Immunocytochemistry
Monoclonal antibodies
Root
β-D-Glucosyl Yariv
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
4
7
2020
pubmed:
4
7
2020
medline:
27
2
2021
Statut:
ppublish
Résumé
Arabinogalactan proteins (AGPs) are important plant proteoglycans involved in many development processes. In roots, AGPs occur in the cell wall of root cells and root cap-derived cells as well as in the secreted mucilage. Detection, localization , and quantification techniques are therefore essential to unravel the AGP diversity of structures and functions. This chapter details root-adapted immunocytochemical methods using monoclonal antibodies, and a collection of biochemical analysis protocols using β-D-glucosyl Yariv reagent for comprehensive AGP characterization.
Identifiants
pubmed: 32617947
doi: 10.1007/978-1-0716-0621-6_22
doi:
Substances chimiques
Glucosides
0
Mucoproteins
0
Plant Mucilage
0
Plant Proteins
0
Yariv reagent
0
arabinogalactan proteins
0
Phloroglucinol
DHD7FFG6YS
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM