LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.
Adolescent
Adult
Aged
Apoptosis
/ genetics
Cell Cycle Checkpoints
/ genetics
Cell Division
/ genetics
Cell Line, Tumor
Cell Proliferation
/ genetics
Epithelial-Mesenchymal Transition
/ genetics
Female
G2 Phase
/ genetics
Humans
Hyaluronan Receptors
/ genetics
Lipase
/ genetics
Lymphatic Metastasis
/ genetics
Middle Aged
Neoplastic Stem Cells
/ pathology
Oxygen Consumption
/ genetics
Triple Negative Breast Neoplasms
/ genetics
Young Adult
epithelial-mesenchymal transition
lipase member H
metastasis
prognosis marker
stem-like cells
triple-negative breast cancer
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
16
10
2019
revised:
05
05
2020
accepted:
07
06
2020
pubmed:
4
7
2020
medline:
7
5
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
Lipase member H (LIPH), a novel member of the triglyceride lipase family. The clinical implications of its expression in breast cancer are still unclear. Therefore, in this study, we investigated the associations between LIPH and the tumorigenic behaviours of 144 triple-negative breast cancer (TNBC) patients. The ratio and mammosphere-forming ability of CD44+/CD24- stem-like cells were tested. The role of LIPH in breast cancer cell migration and invasion was also evaluated. In addition, the effect of LIPH silencing on mitochondrial respiration was determined using the Seahorse assay. Finally, the effect of LIPH silencing on protein expression was determined via tandem mass tag-based spectrometry and Western blotting. We found that LIPH expression was associated with metastasis in lymph nodes and distant organs (P = 0.025), resulting in poor survival among breast cancer patients (P = 0.027). LIPH knockdown significantly decreased both the ratio of CD44
Identifiants
pubmed: 32618099
doi: 10.1111/jcmm.15549
pmc: PMC7417731
doi:
Substances chimiques
Hyaluronan Receptors
0
LIPH protein, human
0
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9125-9134Informations de copyright
© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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