Soybean AP1 homologs control flowering time and plant height.
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
11
05
2020
accepted:
02
07
2020
pubmed:
4
7
2020
medline:
22
6
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
Flowering time and plant height are key agronomic traits that directly affect soybean (Glycine max) yield. APETALA1 (AP1) functions as a class A gene in the ABCE model for floral organ development, helping to specify carpel, stamen, petal, and sepal identities. There are four AP1 homologs in soybean, all of which are mainly expressed in the shoot apex. Here, we used clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR-associated protein 9 technology to generate a homozygous quadruple mutant, gmap1, with loss-of-function mutations in all four GmAP1 genes. Under short-day (SD) conditions, the gmap1 quadruple mutant exhibited delayed flowering, changes in flower morphology, and increased node number and internode length, resulting in plants that were taller than the wild type. Conversely, overexpression of GmAP1a resulted in early flowering and reduced plant height compared to the wild type under SD conditions. The gmap1 mutant and the overexpression lines also exhibited altered expression of several genes related to flowering and gibberellic acid metabolism, thereby providing insight into the role of GmAP1 in the regulatory networks controlling flowering time and plant height in soybean. Increased node number is the trait with the most promise for enhancing soybean pod number and grain yield. Therefore, the mutant alleles of the four AP1 homologs described here will be invaluable for molecular breeding of improved soybean yield.
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1868-1879Subventions
Organisme : National Natural Science Foundation of China
ID : 31901499
Organisme : National Natural Science Foundation of China
ID : 31725021
Organisme : National Natural Science Foundation of China
ID : 31930083
Organisme : National Natural Science Foundation of China
ID : 31801384
Organisme : Major Program of Guangdong Basic and Applied Research
ID : 2019B030302006
Informations de copyright
© 2020 Institute of Botany, Chinese Academy of Sciences.
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