Molecular characteristics of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in Japan: Predominance of CTX-M-15 and emergence of hypervirulent clones.
CTX-M-15
Extended-spectrum β-lactamase
Hypervirulent
Klebsiella pneumoniae
Journal
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
17
05
2020
revised:
22
06
2020
accepted:
24
06
2020
pubmed:
4
7
2020
medline:
11
11
2020
entrez:
4
7
2020
Statut:
ppublish
Résumé
To provide data on the molecular characteristics of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae clinical isolates in Japan. A total of 100 clinical isolates of ESBL-producing K. pneumoniae collected throughout Japan between June and July 2018 were studied. ESBL genes were analyzed using PCR and DNA sequencing. Transferability of ESBL genes was investigated by conjugation experiments. Plasmid replicon types, virulence genes (rmpA, rmpA2, iucA, iroB, and peg-344) associated with hypervirulent K. pneumoniae (hvKp), and capsule types were detected using PCR. Genotyping was performed using multilocus sequence typing. All ESBL-producing isolates carried bla CTX-M-15 was the predominant ESBL among K. pneumoniae isolates from Japan. This study shows that ESBL-producing hvKp strains comprising various clones are emerging in Japan.
Identifiants
pubmed: 32619765
pii: S1201-9712(20)30517-8
doi: 10.1016/j.ijid.2020.06.083
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
281-286Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.