Evaluation of seminal fluid leukocyte subpopulations in patients with varicocele.
Adult
CD3 Complex
/ analysis
CD4-Positive T-Lymphocytes
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Case-Control Studies
Cell Survival
Flow Cytometry
GPI-Linked Proteins
/ analysis
Humans
Immunophenotyping
Leukocyte Common Antigens
/ analysis
Leukocytes
/ immunology
Lipopolysaccharide Receptors
/ analysis
Male
Phenotype
Prospective Studies
Receptors, IgG
/ analysis
Semen
/ immunology
Sperm Count
Spermatozoa
/ pathology
Varicocele
/ immunology
bio-functional sperm parameters
conventional sperm parameters
seminal leukocytes
varicocele
Journal
International journal of immunopathology and pharmacology
ISSN: 2058-7384
Titre abrégé: Int J Immunopathol Pharmacol
Pays: England
ID NLM: 8911335
Informations de publication
Date de publication:
Historique:
entrez:
9
7
2020
pubmed:
9
7
2020
medline:
23
4
2021
Statut:
ppublish
Résumé
Varicocele is a common cause of sperm damage. Some studies showed higher concentration of seminal leukocytes in patients with varicocele. The aim of the study was to evaluate seminal leukocyte subpopulations in patients with varicocele. We enrolled 20 patients with varicocele and 20 age-matched healthy men. Sperm analysis was conducted according to the World Health Organization (WHO) 2010 criteria. We evaluated seminal leukocyte subpopulations and bio-functional sperm parameters by flow cytometry. Patients with varicocele had significantly lower sperm concentration and total number than controls. Regarding seminal leukocyte subpopulations, patients with varicocele had a significantly lower percentage of CD8+ and CD16+ leukocytes and a significantly higher percentage of CD4+ leukocytes than controls. As for bio-functional sperm parameters, we found that patients with varicocele had a significantly lower percentage of alive spermatozoa compared to the control group. These results may explain the increased level of cytokines in the seminal plasma of patients with varicocele.
Identifiants
pubmed: 32635770
doi: 10.1177/2058738420925719
pmc: PMC7359651
doi:
Substances chimiques
CD14 protein, human
0
CD3 Complex
0
FCGR3B protein, human
0
GPI-Linked Proteins
0
Lipopolysaccharide Receptors
0
Receptors, IgG
0
Leukocyte Common Antigens
EC 3.1.3.48
PTPRC protein, human
EC 3.1.3.48
Types de publication
Letter
Langues
eng
Sous-ensembles de citation
IM
Pagination
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