The structures of two archaeal type IV pili illuminate evolutionary relationships.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 07 2020
09 07 2020
Historique:
received:
09
04
2020
accepted:
22
06
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
1
9
2020
Statut:
epublish
Résumé
We have determined the cryo-electron microscopic (cryo-EM) structures of two archaeal type IV pili (T4P), from Pyrobaculum arsenaticum and Saccharolobus solfataricus, at 3.8 Å and 3.4 Å resolution, respectively. This triples the number of high resolution archaeal T4P structures, and allows us to pinpoint the evolutionary divergence of bacterial T4P, archaeal T4P and archaeal flagellar filaments. We suggest that extensive glycosylation previously observed in T4P of Sulfolobus islandicus is a response to an acidic environment, as at even higher temperatures in a neutral environment much less glycosylation is present for Pyrobaculum than for Sulfolobus and Saccharolobus pili. Consequently, the Pyrobaculum filaments do not display the remarkable stability of the Sulfolobus filaments in vitro. We identify the Saccharolobus and Pyrobaculum T4P as host receptors recognized by rudivirus SSRV1 and tristromavirus PFV2, respectively. Our results illuminate the evolutionary relationships among bacterial and archaeal T4P filaments and provide insights into archaeal virus-host interactions.
Identifiants
pubmed: 32647180
doi: 10.1038/s41467-020-17268-4
pii: 10.1038/s41467-020-17268-4
pmc: PMC7347861
doi:
Substances chimiques
Archaeal Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3424Subventions
Organisme : NCRR NIH HHS
ID : G20 RR031199
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122510
Pays : United States
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