Sex-specific features of spine densities in the hippocampus.
Aging
Animals
Animals, Newborn
CA1 Region, Hippocampal
/ cytology
Cells, Cultured
Dendritic Spines
/ ultrastructure
Estrus
Female
Genes, Reporter
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Electron
Primary Cell Culture
Pyramidal Cells
/ ultrastructure
Rats
Rats, Wistar
Receptors, N-Methyl-D-Aspartate
/ analysis
Sex Characteristics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 07 2020
09 07 2020
Historique:
received:
08
11
2018
accepted:
18
06
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
1
1
2021
Statut:
epublish
Résumé
Previously, we found that in dissociated hippocampal cultures the proportion of large spines (head diameter ≥ 0.6 μm) was larger in cultures from female than from male animals. In order to rule out that this result is an in vitro phenomenon, we analyzed the density of large spines in fixed hippocampal vibratome sections of Thy1-GFP mice, in which GFP is expressed only in subpopulations of neurons. We compared spine numbers of the four estrus cycle stages in females with those of male mice. Remarkably, total spine numbers did not vary during the estrus cycle, while estrus cyclicity was evident regarding the number of large spines and was highest during diestrus, when estradiol levels start to rise. The average total spine number in females was identical with the spine number in male animals. The density of large spines, however, was significantly lower in male than in female animals in each stage of the estrus cycle. Interestingly, the number of spine apparatuses, a typical feature of large spines, did not differ between the sexes. Accordingly, NMDA-R1 and NMDA-R2A/B expression were lower in the hippocampus and in postsynaptic density fractions of adult male animals than in those of female animals. This difference could already be observed at birth for NMDA-R1, but not for NMDA-R2A/B expression. In dissociated embryonic hippocampal cultures, no difference was seen after 21 days in culture, while the difference was evident in postnatal cultures. Our data indicate that hippocampal neurons are differentiated in a sex-dependent manner, this differentiation being likely to develop during the perinatal period.
Identifiants
pubmed: 32647191
doi: 10.1038/s41598-020-68371-x
pii: 10.1038/s41598-020-68371-x
pmc: PMC7347548
doi:
Substances chimiques
Receptors, N-Methyl-D-Aspartate
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11405Références
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