"Plug and Play" logic gate construction based on chemically triggered fluorescence switching of gold nanoparticles conjugated with Cy3-tagged aptamer.


Journal

Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782

Informations de publication

Date de publication:
09 07 2020
Historique:
received: 22 03 2020
accepted: 30 06 2020
entrez: 11 7 2020
pubmed: 11 7 2020
medline: 16 6 2021
Statut: epublish

Résumé

Gold nanoparticles (AuNPs) conjugated with Cy3-tagged aptamer which can specifically recognize chloramphenicol (CAP) (referred to as AuNPs-AptCAP) are described. CAP can trigger the configuration change of CAP binding aptamer, and thus switching the fluorescence of AuNPs-AptCAP through changing the efficiency of the fluorescence resonance energy transfer (FRET) system with Cy3 as donors and AuNPs as recipients. AuNPs-AptCAP exhibits a linear range of CAP concentrations from 26.0 to 277 μg L

Identifiants

pubmed: 32647943
doi: 10.1007/s00604-020-04421-5
pii: 10.1007/s00604-020-04421-5
doi:

Substances chimiques

Aptamers, Nucleotide 0
Carbocyanines 0
Fluorescent Dyes 0
Immobilized Nucleic Acids 0
cyanine dye 3 0
Chloramphenicol 66974FR9Q1
Gold 7440-57-5
Nickel 7OV03QG267
DNA 9007-49-2
Edetic Acid 9G34HU7RV0
Mercury FXS1BY2PGL

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

437

Subventions

Organisme : Natural Science Foundation of Guangdong Province
ID : S2017A030313076
Pays : International
Organisme : Guangzhou Municipal Science and Technology Project
ID : 201803020026
Pays : International
Organisme : Shenzhen Technical Project
ID : JCYJ20170307140752183
Pays : International

Auteurs

Yali Zhang (Y)

Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China.

Cheuk-Wing Li (CW)

School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham, NG11 8NS, UK.

Lefei Zhou (L)

Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China.

Zhanpeng Chen (Z)

Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China.

Changqing Yi (C)

Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Biomedical Engineering, Sun Yat-Sen University, Guangzhou, 510006, China. yichq@mail.sysu.edu.cn.
Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen, 518057, China. yichq@mail.sysu.edu.cn.

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Classifications MeSH