A two-lane mechanism for selective biological ammonium transport.

E. coli SSME Saccharomyces cerevisiae ammonium transporter biochemistry chemical biology nitrosomonas europaea rhesus protein transport selectivity

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
14 07 2020
Historique:
received: 24 03 2020
accepted: 13 07 2020
pubmed: 15 7 2020
medline: 24 2 2021
entrez: 15 7 2020
Statut: epublish

Résumé

The transport of charged molecules across biological membranes faces the dual problem of accommodating charges in a highly hydrophobic environment while maintaining selective substrate translocation. This has been the subject of a particular controversy for the exchange of ammonium across cellular membranes, an essential process in all domains of life. Ammonium transport is mediated by the ubiquitous Amt/Mep/Rh transporters that includes the human Rhesus factors. Here, using a combination of electrophysiology, yeast functional complementation and extended molecular dynamics simulations, we reveal a unique two-lane pathway for electrogenic NH

Identifiants

pubmed: 32662768
doi: 10.7554/eLife.57183
pii: 57183
pmc: PMC7447429
doi:
pii:

Substances chimiques

Ammonium Compounds 0
Ammonia 7664-41-7

Banques de données

figshare
['10.6084/m9.figshare.12826316']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Tenovus
ID : S17-07
Pays : International
Organisme : Natural Environment Research Council
ID : NE/M001415/1
Pays : International
Organisme : Fonds De La Recherche Scientifique - FNRS
ID : WELBIO grant ref: CR-2019A-05R.
Pays : International

Commentaires et corrections

Type : CommentIn
Type : ErratumIn

Informations de copyright

© 2020, Williamson et al.

Déclaration de conflit d'intérêts

GW, GT, AB, MB, GD, MB, AP, CI, ET, PH, AM, UZ, AJ No competing interests declared

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Auteurs

Gordon Williamson (G)

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

Giulia Tamburrino (G)

Computational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Physics, School of Science and Engineering, University of Dundee, Dundee, United Kingdom.

Adriana Bizior (A)

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

Mélanie Boeckstaens (M)

Biology of Membrane Transport Laboratory, Department of Molecular Biology, Université Libre de Bruxelles, Gosselies, Belgium.

Gaëtan Dias Mirandela (G)

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

Marcus G Bage (MG)

Computational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Physics, School of Science and Engineering, University of Dundee, Dundee, United Kingdom.

Andrei Pisliakov (A)

Computational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Physics, School of Science and Engineering, University of Dundee, Dundee, United Kingdom.

Callum M Ives (CM)

Computational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.

Eilidh Terras (E)

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

Paul A Hoskisson (PA)

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

Anna Maria Marini (AM)

Biology of Membrane Transport Laboratory, Department of Molecular Biology, Université Libre de Bruxelles, Gosselies, Belgium.

Ulrich Zachariae (U)

Computational Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Physics, School of Science and Engineering, University of Dundee, Dundee, United Kingdom.

Arnaud Javelle (A)

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.

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Classifications MeSH