CRISPR-CasΦ from huge phages is a hypercompact genome editor.

Bacteriophages / genetics CRISPR-Cas Systems Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
17 07 2020
Historique:
received: 05 02 2020
accepted: 06 05 2020
entrez: 18 7 2020
pubmed: 18 7 2020
medline: 11 8 2020
Statut: ppublish

Résumé

CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox.

Identifiants

DOI: 10.1126/science.abb1400 PMID: 32675376 PMC: PMC8207990
pubmed: 32675376
pii: 369/6501/333
doi: 10.1126/science.abb1400
pmc: PMC8207990
mid: NIHMS1702779
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

333-337

Subventions

Organisme : NHGRI NIH HHS
ID : RM1 HG009490
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI142817
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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Auteurs

Patrick Pausch (P)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
ORCID: 0000-0001-8947-9107

Basem Al-Shayeb (B)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA.
ORCID: 0000-0002-3120-3201

Ezra Bisom-Rapp (E)

College of Natural Resources, University of California, Berkeley, Berkeley, CA, USA.

Connor A Tsuchida (CA)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
University of California, Berkeley-University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA.
ORCID: 0000-0002-9563-8052

Zheng Li (Z)

Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles, CA, USA.
ORCID: 0000-0003-2174-7851

Brady F Cress (BF)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
ORCID: 0000-0002-2948-2846

Gavin J Knott (GJ)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia.
ORCID: 0000-0002-9007-6273

Steven E Jacobsen (SE)

Department of Molecular, Cellular and Developmental Biology, University of California, Los Angeles, CA, USA.
Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA.
ORCID: 0000-0001-9483-138X

Jillian F Banfield (JF)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
Department of Earth and Planetary Sciences, University of California, Berkeley, Berkeley, CA, USA.
ORCID: 0000-0001-8203-8771

Jennifer A Doudna (JA)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. doudna@berkeley.edu.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA.
Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Gladstone Institute of Data Science and Biotechnology, Gladstone Institutes, San Francisco, CA, USA.
ORCID: 0000-0001-9161-999X

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Classifications MeSH

questionsmedicales.fr › Virus › Bactériophages › CRISPR-CasΦ from huge phages is a hypercompact...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › CRISPR-CasΦ from huge phages is a hypercompact...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › CRISPR-CasΦ from huge phages is a hypercompact...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › CRISPR-CasΦ from huge phages is a hypercompact...