Platelet activation and platelet-monocyte aggregate formation trigger tissue factor expression in patients with severe COVID-19.


Journal

Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509

Informations de publication

Date de publication:
10 09 2020
Historique:
received: 26 05 2020
accepted: 10 07 2020
pubmed: 18 7 2020
medline: 6 10 2020
entrez: 18 7 2020
Statut: ppublish

Résumé

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emergent pathogen responsible for the coronavirus disease 2019 (COVID-19). Since its emergence, the novel coronavirus has rapidly achieved pandemic proportions causing remarkably increased morbidity and mortality around the world. A hypercoagulability state has been reported as a major pathologic event in COVID-19, and thromboembolic complications listed among life-threatening complications of the disease. Platelets are chief effector cells of hemostasis and pathological thrombosis. However, the participation of platelets in the pathogenesis of COVID-19 remains elusive. This report demonstrates that increased platelet activation and platelet-monocyte aggregate formation are observed in severe COVID-19 patients, but not in patients presenting mild COVID-19 syndrome. In addition, exposure to plasma from severe COVID-19 patients increased the activation of control platelets ex vivo. In our cohort of COVID-19 patients admitted to the intensive care unit, platelet-monocyte interaction was strongly associated with tissue factor (TF) expression by the monocytes. Platelet activation and monocyte TF expression were associated with markers of coagulation exacerbation as fibrinogen and D-dimers, and were increased in patients requiring invasive mechanical ventilation or patients who evolved with in-hospital mortality. Finally, platelets from severe COVID-19 patients were able to induce TF expression ex vivo in monocytes from healthy volunteers, a phenomenon that was inhibited by platelet P-selectin neutralization or integrin αIIb/β3 blocking with the aggregation inhibitor abciximab. Altogether, these data shed light on new pathological mechanisms involving platelet activation and platelet-dependent monocyte TF expression, which were associated with COVID-19 severity and mortality.

Identifiants

pubmed: 32678428
pii: S0006-4971(20)61728-1
doi: 10.1182/blood.2020007252
pmc: PMC7483437
doi:

Substances chimiques

Biomarkers 0
P-Selectin 0
SELP protein, human 0
Thromboplastin 9035-58-9

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1330-1341

Commentaires et corrections

Type : CommentIn
Type : CommentIn

Informations de copyright

© 2020 by The American Society of Hematology.

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Auteurs

Eugenio D Hottz (ED)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Laboratory of Immunothrombosis, Department of Biochemistry, Federal University of Juiz de Fora (UFJF), Juiz de Fora, Minas Gerais, Brazil.

Isaclaudia G Azevedo-Quintanilha (IG)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Lohanna Palhinha (L)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Lívia Teixeira (L)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Ester A Barreto (EA)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Camila R R Pão (CRR)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Cassia Righy (C)

Paulo Niemeyer State Brain Institute, Rio de Janeiro, Brazil.
National Institute of Infectious Disease Evandro Chagas and.

Sérgio Franco (S)

Paulo Niemeyer State Brain Institute, Rio de Janeiro, Brazil.

Thiago M L Souza (TML)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Center for Technological Development in Health (CDTS), Oswaldo Cruz Foundation, Rio de Janeiro, Brazil; and.

Pedro Kurtz (P)

Paulo Niemeyer State Brain Institute, Rio de Janeiro, Brazil.
D'Or Institute for Research and Education, Rio de Janeiro, Brazil.

Fernando A Bozza (FA)

National Institute of Infectious Disease Evandro Chagas and.
D'Or Institute for Research and Education, Rio de Janeiro, Brazil.

Patrícia T Bozza (PT)

Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

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