Enhancement of glucaric acid production in Saccharomyces cerevisiae by expressing Vitreoscilla hemoglobin.
Glucaric acid
Saccharomyces cerevisiae
Vitreoscilla hemoglobin
pH-free fermentation
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
26
03
2020
accepted:
11
07
2020
pubmed:
22
7
2020
medline:
6
7
2021
entrez:
22
7
2020
Statut:
ppublish
Résumé
To enhance the glucaric acid (GA) production in Saccharomyces cerevisiae, the Vitreoscilla hemoglobin was employed to reinforce cellular oxygen supplement. Additionally, the pH-free fermentation strategy was engaged to lower the cost brought by base feeding during the acid-accumulated and long-period glucaric acid production. Recombinant yeast Bga-4 was constructed harboring Vitreoscilla hemoglobin on the basis of previous Bga-3. Higher glucose uptake rate, growth rate, and ethanol reuse rate were achieved in Bga-4 in shake-flask fermentation than those in Bga-3. Furthermore, the fed-batch fermentation in a 5-L bioreactor was performed without pH control, resulting in a final glucaric acid titer of 6.38 g/L. Both the GA titer and biomass were enhanced along with the efficiency of ethanol re-utilization in the presence of VHb. Moreover, the absence of base feeding for long-period fermentation reduced production cost, which is meaningful for industrial applications.
Identifiants
pubmed: 32691185
doi: 10.1007/s10529-020-02966-2
pii: 10.1007/s10529-020-02966-2
doi:
Substances chimiques
Bacterial Proteins
0
Recombinant Proteins
0
Truncated Hemoglobins
0
hemoglobin protein, Vitreoscilla
104781-86-4
Glucaric Acid
QLZ991V4A2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2169-2178Subventions
Organisme : the National Key R&D Program of China
ID : 2019YFA0905502
Organisme : the National Natural Science Foundation of China
ID : 21877053
Organisme : the National Natural Science Foundation of China
ID : 31601564
Organisme : the Natural Science Foundation of Jiangsu Province
ID : BK20181345
Organisme : the National First-class Discipline Program of Light Industry Technology and Engineering
ID : LITE2018-24
Organisme : the Open Foundation of Jiangsu Key Laboratory of Industrial Biotechnology
ID : KLIB-KF201807
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