Synergistic effects of Cinnamomum cassia L. essential oil in combination with polymyxin B against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens.
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ antagonists & inhibitors
Cinnamomum aromaticum
/ chemistry
Drug Resistance, Multiple, Bacterial
/ drug effects
Drug Synergism
Humans
Klebsiella Infections
/ drug therapy
Klebsiella pneumoniae
/ drug effects
Oils, Volatile
/ pharmacology
Polymyxin B
/ pharmacology
Serratia Infections
/ drug therapy
Serratia marcescens
/ drug effects
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
beta-Lactamases
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
21
02
2020
accepted:
07
07
2020
entrez:
24
7
2020
pubmed:
24
7
2020
medline:
25
9
2020
Statut:
epublish
Résumé
Multidrug resistance prompts the search for new sources of antibiotics with new targets at bacteria cell. To investigate the antibacterial activity of Cinnamomum cassia L. essential oil (CCeo) alone and in combination with antibiotics against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens. The antimicrobial susceptibility of the strains was determined by Vitek® 2 and confirmed by MALDI-TOF/TOF. The antibacterial activity of CCeo and its synergism with antibiotics was determined using agar disk diffusion, broth microdilution, time-kill, and checkboard methods. The integrity of the bacterial cell membrane in S. marcescens was monitored by protein leakage assay. CCeo exhibited inhibitory effects with MIC = 281.25 μg.mL-1. The association between CCeo and polymyxin B showed a decrease in terms of viable cell counts on survival curves over time after a 4 hour-treatment with a FIC index value of 0.006. Protein leakage was observed with increasing concentrations for CCeo and CCeo + polymyxin B treatments. CCeo showed antibacterial activity against the studied strains. When associated with polymyxin B, a synergistic effect was able to inhibit bacterial growth rapidly and consistently, making it a potential candidate for the development of an alternative treatment and drug delivery system for carbapenemase-producing strains.
Identifiants
pubmed: 32701970
doi: 10.1371/journal.pone.0236505
pii: PONE-D-20-05109
pmc: PMC7377461
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Oils, Volatile
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Polymyxin B
J2VZ07J96K
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0236505Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Antimicrob Chemother. 2006 Jan;57(1):154-5
pubmed: 16284100
Pharmaceuticals (Basel). 2013 Nov 25;6(12):1451-74
pubmed: 24287491
Braz J Infect Dis. 2019 Jan - Feb;23(1):60-65
pubmed: 30796888
Pharmaceutics. 2020 May 07;12(5):
pubmed: 32392726
Mem Inst Oswaldo Cruz. 2019;114:e190020
pubmed: 31166421
J Clin Microbiol. 2009 Feb;47(2):322-6
pubmed: 19036932
Appl Microbiol Biotechnol. 2016 Nov;100(22):9619-9627
pubmed: 27562470
Front Microbiol. 2014 Nov 26;5:643
pubmed: 25505462
J Microbiol Methods. 2014 Dec;107:106-18
pubmed: 25304059
Jundishapur J Microbiol. 2016 Jan 02;9(1):e29314
pubmed: 27099689
Can J Microbiol. 2015 Apr;61(4):263-71
pubmed: 25728340
Food Chem Toxicol. 2005 Jun;43(6):799-836
pubmed: 15811570
Int J Antimicrob Agents. 2006 Apr;27(4):351-3
pubmed: 16564159
Food Chem Toxicol. 1988 Jun;26(6):487-500
pubmed: 3169648
J Clin Microbiol. 2013 Jan;51(1):287-90
pubmed: 23100344
J Microbiol Biotechnol. 2015 Aug;25(8):1299-306
pubmed: 25381741
Microb Pathog. 2018 Jul;120:198-203
pubmed: 29702210
Antimicrob Agents Chemother. 2014 Sep;58(9):5181-90
pubmed: 24957827
J Med Microbiol. 2014 Oct;63(Pt 10):1303-1310
pubmed: 25060972
Food Chem Toxicol. 1994 Oct;32(10):869-76
pubmed: 7959441
Lett Appl Microbiol. 2016 Nov;63(5):322-334
pubmed: 27542872
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
pubmed: 14665678
J Med Microbiol. 2016 Jun;65(6):547-553
pubmed: 27002853
Antimicrob Agents Chemother. 2016 Apr 22;60(5):3001-6
pubmed: 26953205
J Pharm Sci. 2015 Mar;104(3):906-15
pubmed: 25561249
Planta Med. 2011 Jul;77(11):1168-82
pubmed: 21283954
Eur J Clin Pharmacol. 2015 Jul;71(7):801-10
pubmed: 26008213
J Oleo Sci. 2015;64(3):289-98
pubmed: 25757433
J Microencapsul. 2018 Jun;35(4):344-356
pubmed: 29889598
J Clin Microbiol. 2015 Jul;53(7):2324-8
pubmed: 25878341
Lancet Infect Dis. 2016 Feb;16(2):161-8
pubmed: 26603172
Curr Med Res Opin. 2015 Apr;31(4):707-21
pubmed: 25697677
Am J Infect Control. 2016 Nov 1;44(11):1374-1380
pubmed: 27156198