Ex vivo magnetic particle imaging of vascular inflammation in abdominal aortic aneurysm in a murine model.

Angiotensin II / toxicity Animals Aorta, Abdominal / diagnostic imaging Aortic Aneurysm, Abdominal / chemically induced Disease Models, Animal Disease Progression Feasibility Studies Humans Inflammation Magnetic Iron Oxide Nanoparticles / administration & dosage Magnetic Resonance Angiography / methods Magnetic Resonance Imaging Magnetic Resonance Spectroscopy / methods Male Mice Mice, Knockout, ApoE

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 07 2020
Historique:
received: 05 05 2020
accepted: 26 06 2020
entrez: 26 7 2020
pubmed: 28 7 2020
medline: 22 12 2020
Statut: epublish

Résumé

Abdominal aortic aneurysms (AAAs) are currently one of the leading causes of death in developed countries. Inflammation is crucial in the disease progression, having a substantial impact on various determinants in AAAs development. Magnetic particle imaging (MPI) is an innovative imaging modality, enabling the highly sensitive detection of magnetic nanoparticles (MNPs), suitable as surrogate marker for molecular targeting of vascular inflammation. For this study, Apolipoprotein E-deficient-mice underwent surgical implantation of osmotic minipumps with constant Angiotensin II infusion. After 3 and 4 weeks respectively, in-vivo-magnetic resonance imaging (MRI), ex-vivo-MPI and ex-vivo-magnetic particle spectroscopy (MPS) were performed. The results were validated by histological analysis, immunohistology and laser ablation-inductively coupled plasma-mass spectrometry. MR-angiography enabled the visualization of aneurysmal development and dilatation in the experimental group. A close correlation (R = 0.87) with histological area assessment was measured. Ex-vivo-MPS revealed abundant iron deposits in AAA samples and ex-vivo histopathology measurements were in good agreement (R = 0.76). Ex-vivo-MPI and MPS results correlated greatly (R = 0.99). CD68-immunohistology stain and Perls'-Prussian-Blue-stain confirmed the colocalization of macrophages and MNPs. This study demonstrates the feasibility of ex-vivo-MPI for detecting inflammation in AAA. The quantitative ability for mapping MNPs establishes MPI as a promising tool for monitoring inflammatory progression in AAA in an experimental setting.

Identifiants

DOI: 10.1038/s41598-020-69299-y PMID: 32709967 PMC: PMC7381631
pubmed: 32709967
doi: 10.1038/s41598-020-69299-y
pii: 10.1038/s41598-020-69299-y
pmc: PMC7381631
doi:

Substances chimiques

Angiotensin II 11128-99-7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

12410

Références

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Auteurs

Dilyana B Mangarova (DB)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany. dilyana.mangarova@charite.de.
Department of Veterinary Medicine, Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, Building 12, 14163, Berlin, Germany. dilyana.mangarova@charite.de.

Julia Brangsch (J)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany.
Department of Veterinary Medicine, Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Freie Universität Berlin, Königsweg 67, Building 21, 14163, Berlin, Germany.

Azadeh Mohtashamdolatshahi (A)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany.

Olaf Kosch (O)

Department 8.2-Biosignals, Physikalisch-Technische Bundesanstalt Berlin, Abbestrasse 2-12, 10587, Berlin, Germany.

Hendrik Paysen (H)

Department 8.2-Biosignals, Physikalisch-Technische Bundesanstalt Berlin, Abbestrasse 2-12, 10587, Berlin, Germany.

Frank Wiekhorst (F)

Department 8.2-Biosignals, Physikalisch-Technische Bundesanstalt Berlin, Abbestrasse 2-12, 10587, Berlin, Germany.

Robert Klopfleisch (R)

Department of Veterinary Medicine, Institute of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str. 15, Building 12, 14163, Berlin, Germany.

Rebecca Buchholz (R)

Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 30, 48149, Münster, Germany.

Uwe Karst (U)

Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 30, 48149, Münster, Germany.

Matthias Taupitz (M)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany.

Jörg Schnorr (J)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany.

Bernd Hamm (B)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany.

Marcus R Makowski (MR)

Department of Radiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Charitéplatz 1, 10117, Berlin, Germany.
Department of Diagnostic and Interventional Radiology, Technische Universität München, Ismaninger Str. 22, 81675, Munich, Germany.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Médiateurs de l'inflammation › Autacoïdes › Angiotensines › Angiotensine-II › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Eucaryotes › Animaux › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Système cardiovasculaire › Vaisseaux sanguins › Artères › Aorte › Aorte abdominale › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Maladies de l'aorte › Anévrysme de l'aorte › Anévrysme de l'aorte abdominale › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Caractéristiques de la maladie › Évolution de la maladie › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études de faisabilité › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Inflammation › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Nanoparticules métalliques › Nanoparticules magnétiques d'oxyde de fer › Ex vivo magnetic particle imaging of vascular...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Techniques de diagnostic cardiovasculaire › Angiographie › Angiographie par résonance magnétique › Ex vivo magnetic particle imaging of vascular...
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