Preferential inhibition of adaptive immune system dynamics by glucocorticoids in patients after acute surgical trauma.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
27 07 2020
Historique:
received: 21 10 2019
accepted: 03 07 2020
entrez: 29 7 2020
pubmed: 29 7 2020
medline: 9 9 2020
Statut: epublish

Résumé

Glucocorticoids (GC) are a controversial yet commonly used intervention in the clinical management of acute inflammatory conditions, including sepsis or traumatic injury. In the context of major trauma such as surgery, concerns have been raised regarding adverse effects from GC, thereby necessitating a better understanding of how GCs modulate the immune response. Here we report the results of a randomized controlled trial (NCT02542592) in which we employ a high-dimensional mass cytometry approach to characterize innate and adaptive cell signaling dynamics after a major surgery (primary outcome) in patients treated with placebo or methylprednisolone (MP). A robust, unsupervised bootstrap clustering of immune cell subsets coupled with random forest analysis shows profound (AUC = 0.92, p-value = 3.16E-8) MP-induced alterations of immune cell signaling trajectories, particularly in the adaptive compartments. By contrast, key innate signaling responses previously associated with pain and functional recovery after surgery, including STAT3 and CREB phosphorylation, are not affected by MP. These results imply cell-specific and pathway-specific effects of GCs, and also prompt future studies to examine GCs' effects on clinical outcomes likely dependent on functional adaptive immune responses.

Identifiants

pubmed: 32719355
doi: 10.1038/s41467-020-17565-y
pii: 10.1038/s41467-020-17565-y
pmc: PMC7385146
doi:

Substances chimiques

Glucocorticoids 0
STAT3 Transcription Factor 0
STAT3 protein, human 0
NF-KappaB Inhibitor alpha 139874-52-5
Methylprednisolone X4W7ZR7023

Banques de données

ClinicalTrials.gov
['NCT02542592']

Types de publication

Journal Article Randomized Controlled Trial Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3737

Subventions

Organisme : NIGMS NIH HHS
ID : K23 GM111657
Pays : United States
Organisme : NCATS NIH HHS
ID : KL2 TR003143
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM137936
Pays : United States
Organisme : NINDS NIH HHS
ID : R61 NS114926
Pays : United States

Commentaires et corrections

Type : ErratumIn

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Auteurs

Edward A Ganio (EA)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Natalie Stanley (N)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Viktoria Lindberg-Larsen (V)

The Lundbeck Foundation Center for Fast-track Hip and Knee replacement, Copenhagen, Denmark.

Jakob Einhaus (J)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Amy S Tsai (AS)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Franck Verdonk (F)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Anthony Culos (A)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Sajjad Ghaemi (S)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.
Digital Technologies Research Centre, National Research Council Canada, Toronto, ON, Canada.

Kristen K Rumer (KK)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Ina A Stelzer (IA)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Dyani Gaudilliere (D)

Division of Plastic and Reconstructive Surgery, Department of Surgery, School of Medicine, Stanford University, Stanford, CA, USA.

Eileen Tsai (E)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Ramin Fallahzadeh (R)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Benjamin Choisy (B)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Henrik Kehlet (H)

Section of Surgical Pathophysiology 7621, Rigshospitalet, Blegdamsvej 9, DK-2100, Copenhagen, Denmark.

Nima Aghaeepour (N)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Martin S Angst (MS)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA.

Brice Gaudilliere (B)

Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, CA, USA. gbrice@stanford.edu.

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