A programmable sequence of reporters for lineage analysis.
Journal
Nature neuroscience
ISSN: 1546-1726
Titre abrégé: Nat Neurosci
Pays: United States
ID NLM: 9809671
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
26
07
2019
accepted:
19
06
2020
pubmed:
29
7
2020
medline:
9
2
2021
entrez:
29
7
2020
Statut:
ppublish
Résumé
We present CLADES (cell lineage access driven by an edition sequence), a technology for cell lineage studies based on CRISPR-Cas9 techniques. CLADES relies on a system of genetic switches to activate and inactivate reporter genes in a predetermined order. Targeting CLADES to progenitor cells allows the progeny to inherit a sequential cascade of reporters, thereby coupling birth order to reporter expression. This system, which can also be temporally induced by heat shock, enables the temporal resolution of lineage development and can therefore be used to deconstruct an extended cell lineage by tracking the reporters expressed in the progeny. When targeted to the germ line, the same cascade progresses across animal generations, predominantly marking each generation with the corresponding combination of reporters. CLADES therefore offers an innovative strategy for making programmable cascades of genes that can be used for genetic manipulation or to record serial biological events.
Identifiants
pubmed: 32719561
doi: 10.1038/s41593-020-0676-9
pii: 10.1038/s41593-020-0676-9
doi:
Substances chimiques
Heat-Shock Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1618-1628Subventions
Organisme : NIH HHS
ID : P40 OD010949
Pays : United States
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Commentaires et corrections
Type : CommentIn
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