Identification and validation of a novel candidate gene regulating net meat weight in Simmental beef cattle based on imputed next-generation sequencing.
Animals
Cattle
/ genetics
Cells, Cultured
Genome-Wide Association Study
Haplotypes
High-Throughput Nucleotide Sequencing
Intercellular Signaling Peptides and Proteins
/ genetics
Linkage Disequilibrium
Muscles
/ metabolism
Phenotype
Polymorphism, Single Nucleotide
Quantitative Trait Loci
Red Meat
/ analysis
Differentiation
GWAS
NGS
NMW
Proliferation
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
09
04
2020
revised:
12
06
2020
accepted:
13
06
2020
pubmed:
30
7
2020
medline:
29
12
2020
entrez:
30
7
2020
Statut:
ppublish
Résumé
Genome-wide association studies (GWAS) represent a powerful approach to detecting candidate genes for economically important traits in livestock. Our aim was to identify promising candidate muscle development genes that affect net meat weight (NMW) and validate these candidate genes in cattle. Using a next-generation sequencing (NGS) dataset, we applied ~ 12 million imputed single nucleotide polymorphisms (SNPs) from 1,252 Simmental cattle to detect genes influencing net meat yield by way of a linear mixed model method. Haplotype and linkage disequilibrium (LD) blocks were employed to augment support for identified genes. To investigate the role of MTPN in bovine muscle development, we isolated myoblasts from the longissimus dorsi of a bovine foetus and treated the cells during proliferation, differentiation and hypertrophy. We identified one SNP (rs100670823) that exceeded our stringent significance threshold (P = 8.58 × 10 The present experiments showed that myotrophin increases differentiation and hypertrophy of skeletal muscle cells, while inhibiting their proliferation. Our examination of GWAS results with in vitro biological studies provides new information regarding the potential application of myotrophin to increase meat yields in cattle and helpful information for further studies.
Identifiants
pubmed: 32722873
doi: 10.1111/cpr.12870
pmc: PMC7507581
doi:
Substances chimiques
Intercellular Signaling Peptides and Proteins
0
myotrophin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12870Subventions
Organisme : Cattle Breeding Innovative Research Team
ID : ASTIPIAS03
Organisme : Cattle Breeding Innovative Research Team
ID : ZDXT2018006
Informations de copyright
© 2020 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
Références
Am J Hum Genet. 2017 Jul 6;101(1):5-22
pubmed: 28686856
Cell Prolif. 2018 Oct;51(5):e12473
pubmed: 30094882
Nat Genet. 2009 Oct;41(10):1122-6
pubmed: 19767754
Meat Sci. 2004 Jun;67(2):195-201
pubmed: 22061314
Mol Biol Rep. 2013 Feb;40(2):1947-54
pubmed: 23143182
Genet Mol Res. 2014 Apr 14;13(2):2772-83
pubmed: 24782091
Breast Cancer Res. 2009;11 Suppl 3:S12
pubmed: 20030863
J Cell Biol. 2008 Feb 25;180(4):787-802
pubmed: 18299349
Animals (Basel). 2019 Oct 24;9(11):
pubmed: 31652908
Anim Genet. 2009 Feb;40(1):27-34
pubmed: 19016677
FEBS J. 2018 Oct;285(20):3688-3694
pubmed: 29761627
Anim Genet. 2017 Jun;48(3):369-370
pubmed: 28116762
BMC Genomics. 2011 Aug 11;12:408
pubmed: 21831322
J Biol Chem. 1990 Sep 25;265(27):16635-43
pubmed: 2144530
BMC Genet. 2011 Dec 14;12:103
pubmed: 22168586
Int J Biochem Cell Biol. 2001 Aug;33(8):831-8
pubmed: 11404186
Anim Genet. 2010 Dec;41(6):597-607
pubmed: 20477797
J Mol Cell Cardiol. 1999 Apr;31(4):705-19
pubmed: 10329199
Am J Hum Genet. 2016 Jan 7;98(1):116-26
pubmed: 26748515
BMC Genet. 2009 Aug 04;10:43
pubmed: 19653884
Int J Biochem Cell Biol. 2006;38(7):1114-22
pubmed: 16531094
FEBS Lett. 2012 Sep 21;586(19):3485-92
pubmed: 22967900
Bioinformatics. 2015 Mar 1;31(5):782-4
pubmed: 25338720
Nat Rev Genet. 2009 May;10(5):318-29
pubmed: 19373277
Cell Prolif. 2020 Sep;53(9):e12870
pubmed: 32722873
DNA Res. 2015 Aug;22(4):259-67
pubmed: 26117497
Int J Epidemiol. 2008 Feb;37(1):120-32
pubmed: 17898028
Nat Rev Genet. 2009 Jun;10(6):381-91
pubmed: 19448663
Nat Rev Genet. 2005 Feb;6(2):95-108
pubmed: 15716906
Bioinformatics. 2005 Jan 15;21(2):263-5
pubmed: 15297300
Cell. 1978 Apr;13(4):599-611
pubmed: 657269
Nat Genet. 2010 Nov;42(11):931-6
pubmed: 20972442
J Clin Psychiatry. 2007 Apr;68(4):613-8
pubmed: 17474819
Meat Sci. 2003 Apr;63(4):491-500
pubmed: 22062519
PLoS One. 2019 Oct 10;14(10):e0223671
pubmed: 31600309
Sci Rep. 2017 Aug 2;7(1):7168
pubmed: 28769032
Plant Physiol. 2017 Feb;173(2):921-931
pubmed: 28057894
Biotechniques. 2000 Aug;29(2):332-7
pubmed: 10948434
Methods Cell Biol. 1997;52:155-76
pubmed: 9379949
Cell Prolif. 2019 May;52(3):e12600
pubmed: 30945361
Int J Biochem Cell Biol. 1998 Aug;30(8):897-908
pubmed: 9744081
Am J Hum Genet. 2007 Sep;81(3):559-75
pubmed: 17701901
J Cell Biol. 2002 Dec 23;159(6):1019-28
pubmed: 12486112
Int J Mol Sci. 2020 Feb 13;21(4):
pubmed: 32070024
Bioinformatics. 2007 May 15;23(10):1294-6
pubmed: 17384015
BMC Genomics. 2014 Mar 27;15:240
pubmed: 24673797
Mamm Genome. 2007 Feb;18(2):125-36
pubmed: 17347893
BMC Genet. 2012 May 20;13:40
pubmed: 22607022
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Mol Ther. 2012 Mar;20(3):661-8
pubmed: 22158056
BMC Dev Biol. 2010 Feb 22;10:21
pubmed: 20175910
Nat Rev Genet. 2019 Aug;20(8):467-484
pubmed: 31068683
Meat Sci. 2004 Jul;67(3):463-70
pubmed: 22061521
Mol Biol Rep. 2012 Mar;39(3):2733-8
pubmed: 21667249
Biol Reprod. 2010 Jan;82(1):4-12
pubmed: 19516021