Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace.
Animals
DNA, Ancient
/ isolation & purification
DNA, Protozoan
/ genetics
DNA, Ribosomal
/ genetics
Geologic Sediments
/ parasitology
High-Throughput Nucleotide Sequencing
History, 19th Century
Host Specificity
Humans
Intestinal Diseases, Parasitic
/ history
Italy
Metagenomics
/ methods
RNA, Ribosomal, 18S
/ genetics
Sequence Analysis, DNA
Trichuriasis
/ history
Trichuris
/ classification
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 07 2020
29 07 2020
Historique:
received:
03
07
2019
accepted:
08
07
2020
entrez:
31
7
2020
pubmed:
31
7
2020
medline:
17
12
2020
Statut:
epublish
Résumé
This study evidenced the presence of parasites in a cesspit of an aristocratic palace of nineteenth century in Sardinia (Italy) by the use of classical paleoparasitological techniques coupled with next-generation sequencing. Parasite eggs identified by microscopy included helminth genera pathogenic for humans and animals: the whipworm Trichuris sp., the roundworm Ascaris sp., the flatworm Dicrocoelium sp. and the fish tapeworm Diphyllobothrium sp. In addition, 18S rRNA metabarcoding and metagenomic sequencing analysis allowed the first description in Sardinia of aDNA of the human specific T. trichiura species and Ascaris genus. Their presence is important for understanding the health conditions, hygiene habits, agricultural practices and the diet of the local inhabitants in the period under study.
Identifiants
pubmed: 32728085
doi: 10.1038/s41598-020-69497-8
pii: 10.1038/s41598-020-69497-8
pmc: PMC7391740
doi:
Substances chimiques
DNA, Ancient
0
DNA, Protozoan
0
DNA, Ribosomal
0
RNA, Ribosomal, 18S
0
Types de publication
Historical Article
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12656Références
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