Accelerated Development of Rod Photoreceptors in Retinal Organoids Derived from Human Pluripotent Stem Cells by Supplementation with 9-
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
19 06 2020
19 06 2020
Historique:
entrez:
31
7
2020
pubmed:
31
7
2020
medline:
31
7
2020
Statut:
ppublish
Résumé
Human pluripotent stem cells (PSCs) can be differentiated into retinal organoids with proper neural layer organization, yet the protocols are technically challenging and time consuming. We have modified a widely used differentiation protocol by switching all-
Identifiants
pubmed: 32728670
doi: 10.1016/j.xpro.2020.100033
pmc: PMC7388645
mid: NIHMS1612599
pii:
doi:
Substances chimiques
Diterpenes
0
9-cis-retinal
514-85-2
Retinaldehyde
RR725D715M
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Subventions
Organisme : Intramural NIH HHS
ID : ZIA EY000450
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA EY000474
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA EY000546
Pays : United States
Déclaration de conflit d'intérêts
DECLARATION OF INTERESTS The authors declare no competing interests.
Références
Nat Commun. 2014 Jun 10;5:4047
pubmed: 24915161
Mol Vis. 2019 Nov 11;25:663-678
pubmed: 31814692
Mol Vis. 2020 Mar 03;26:97-105
pubmed: 32174751