The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley.
Sr22
Sr33
Sr35
Sr45
Ug99
barley
durable disease resistance
stacking
stem rust
wheat
Journal
Plant biotechnology journal
ISSN: 1467-7652
Titre abrégé: Plant Biotechnol J
Pays: England
ID NLM: 101201889
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
12
07
2018
accepted:
17
06
2020
pubmed:
4
8
2020
medline:
22
4
2021
entrez:
4
8
2020
Statut:
ppublish
Résumé
In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley's genetic variation for stem rust resistance is very narrow with only ten resistance genes genetically identified. Of these, only one complex locus consisting of three genes is effective against TTKSK, a widely virulent Pgt race of the Ug99 tribe which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Furthermore, these transgenic plants did not display significant adverse agronomic effects in the absence of disease. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.
Identifiants
pubmed: 32744350
doi: 10.1111/pbi.13460
pmc: PMC7868974
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
273-284Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P016855/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L011794/1
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Références
Science. 2013 Aug 16;341(6147):746-51
pubmed: 23950531
Theor Appl Genet. 2018 Oct;131(10):2245-2266
pubmed: 30109391
Theor Appl Genet. 2005 Aug;111(3):496-504
pubmed: 15918008
Plant J. 2015 Oct;84(1):202-15
pubmed: 26315512
Plant J. 2001 Feb;25(3):335-48
pubmed: 11208025
PLoS One. 2013;8(2):e55954
pubmed: 23437080
Phytopathology. 2009 Apr;99(4):339-43
pubmed: 19271974
PLoS Genet. 2018 Apr 3;14(4):e1007287
pubmed: 29614079
Front Plant Sci. 2014 Dec 04;5:692
pubmed: 25538723
Plant Methods. 2019 Oct 24;15:118
pubmed: 31666804
Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):E9483-E9492
pubmed: 29078294
New Phytol. 2010 Jan;185(1):285-99
pubmed: 19814776
Plant Cell. 2018 Feb;30(2):285-299
pubmed: 29382771
Nat Genet. 2015 Dec;47(12):1494-8
pubmed: 26551671
Plant Biotechnol J. 2017 Jul;15(7):894-905
pubmed: 28005310
Curr Opin Plant Biol. 2011 Aug;14(4):468-76
pubmed: 21531167
Annu Rev Genet. 2017 Nov 27;51:435-454
pubmed: 28934591
Theor Appl Genet. 2018 Mar;131(3):625-635
pubmed: 29164273
Genome Biol. 2016 Oct 31;17(1):221
pubmed: 27795210
Theor Appl Genet. 2014 Jun;127(6):1293-304
pubmed: 24710821
Plant Biotechnol J. 2017 Apr;15(4):489-496
pubmed: 27734576
PLoS One. 2011 Feb 18;6(2):e16765
pubmed: 21364738
Plant Methods. 2008 Sep 26;4:22
pubmed: 18822125
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15383-8
pubmed: 16230639
Methods Mol Biol. 2019;1900:115-126
pubmed: 30460562
Phytopathology. 2011 Dec;101(12):1418-23
pubmed: 22070278
Nat Biotechnol. 2019 Feb;37(2):139-143
pubmed: 30718880
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14970-5
pubmed: 18812501
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9328-33
pubmed: 12077318
Front Plant Sci. 2014 Nov 24;5:641
pubmed: 25505474
Mol Plant Microbe Interact. 2020 Feb;33(2):308-319
pubmed: 31556346
Cell. 1994 Sep 23;78(6):1089-99
pubmed: 7923358
Nat Biotechnol. 2016 Jun;34(6):661-5
pubmed: 27111723
Mol Plant Microbe Interact. 2013 Apr;26(4):407-18
pubmed: 23216085
Science. 2013 Aug 16;341(6147):783-786
pubmed: 23811222
Nat Biotechnol. 2017 Aug;35(8):793-796
pubmed: 28504667
Front Plant Sci. 2014 Nov 25;5:606
pubmed: 25506347
Theor Appl Genet. 2016 Apr;129(4):831-843
pubmed: 26754419
Phytopathology. 2013 Nov;103(11):1153-61
pubmed: 23841622
Science. 2002 Apr 26;296(5568):744-7
pubmed: 11976458
Theor Appl Genet. 2017 Jun;130(6):1207-1222
pubmed: 28275817
PLoS One. 2008;3(11):e3647
pubmed: 18985154
Nat Biotechnol. 2016 Jun;34(6):652-5
pubmed: 27111722
Theor Appl Genet. 2014 Apr;127(4):969-80
pubmed: 24504553
Plant Biotechnol J. 2016 May;14(5):1261-8
pubmed: 26471973
Plant Cell. 2004 Mar;16(3):755-68
pubmed: 14973158
Annu Rev Phytopathol. 2002;40:349-79
pubmed: 12147764
Science. 2013 Aug 16;341(6147):786-8
pubmed: 23811228
Plant Biotechnol J. 2017 Nov;15(11):1387-1396
pubmed: 28301718
Plant Cell. 2008 Aug;20(8):2009-17
pubmed: 18723576
Curr Opin Plant Biol. 2017 Aug;38:59-67
pubmed: 28494248
Curr Opin Plant Biol. 2008 Apr;11(2):187-92
pubmed: 18343713
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10300-5
pubmed: 9707642
Phytopathology. 2015 Jul;105(7):917-28
pubmed: 25775107
Plant Cell. 2005 Apr;17(4):1292-305
pubmed: 15749765
BMC Plant Biol. 2016 Jan 27;16 Suppl 1:6
pubmed: 26821649
Cell Host Microbe. 2011 Feb 17;9(2):125-36
pubmed: 21320695
Plant Dis. 2000 Feb;84(2):203
pubmed: 30841334
Nat Rev Genet. 2010 Aug;11(8):539-48
pubmed: 20585331
G3 (Bethesda). 2017 Oct 5;7(10):3491-3507
pubmed: 28855281
Nature. 2017 Feb 2;542(7640):145-146
pubmed: 28179687
Plant Biotechnol J. 2013 Sep;11(7):847-54
pubmed: 23711079
New Phytol. 2019 Jul;223(2):853-866
pubmed: 30913300
Nat Plants. 2015 Nov 30;1:15186
pubmed: 27251721
Theor Appl Genet. 1992 Oct;85(1):73-8
pubmed: 24197231
Theor Appl Genet. 2018 Jan;131(1):107-126
pubmed: 29177535
Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):364-9
pubmed: 12509512
Phytopathology. 2017 Aug;107(8):950-962
pubmed: 28398875
Plant Biotechnol J. 2021 Feb;19(2):273-284
pubmed: 32744350
Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14153-8
pubmed: 10570214
Plant Dis. 2008 Jun;92(6):923-926
pubmed: 30769714
PLoS One. 2016 Jun 16;11(6):e0157029
pubmed: 27309724
Theor Appl Genet. 2005 Dec;112(1):41-50
pubmed: 16283230
Annu Rev Phytopathol. 2015;53:565-89
pubmed: 26047566
Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2531-6
pubmed: 17277084
Nat Protoc. 2018 Dec;13(12):2944-2963
pubmed: 30446746