Selfing mutants link Ku proteins to mating type determination in Tetrahymena.
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
04
04
2019
accepted:
07
07
2020
entrez:
4
8
2020
pubmed:
4
8
2020
medline:
18
9
2020
Statut:
epublish
Résumé
Recognition of self and nonself is important for outcrossing organisms, and different mating types establish the barrier against self-mating. In the unicellular ciliate T. thermophila, mating type determination requires complex DNA rearrangements at a single mat locus during conjugation to produce a type-specific gene pair (MTA and MTB) for 1 of 7 possible mating types. Surprisingly, we found that decreased expression of the DNA breakage-repair protein Ku80 at late stages of conjugation generated persistent selfing phenotype in the progeny. DNA analysis revealed multiple mating-type gene pairs as well as a variety of mis-paired, unusually arranged mating-type genes in these selfers that resemble some proposed rearrangement intermediates. They are found also in normal cells during conjugation and are lost after 10 fissions but are retained in Ku mutants. Silencing of TKU80 or TKU70-2 immediately after conjugation also generated selfing phenotype, revealing a hidden DNA rearrangement process beyond conjugation. Mating reactions between the mutant and normal cells suggest a 2-component system for self-nonself-recognition through MTA and MTB genes.
Identifiants
pubmed: 32745139
doi: 10.1371/journal.pbio.3000756
pii: PBIOLOGY-D-19-00965
pmc: PMC7398496
doi:
Substances chimiques
DNA, Protozoan
0
Protozoan Proteins
0
Ku Autoantigen
EC 4.2.99.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000756Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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