Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise.
Alanine
/ administration & dosage
Amino Acids, Branched-Chain
/ administration & dosage
Animals
Dietary Supplements
Isoleucine
/ administration & dosage
Leucine
/ administration & dosage
Mice
Models, Animal
Muscle Fatigue
/ drug effects
Muscle Proteins
/ metabolism
Muscle, Skeletal
/ drug effects
Performance-Enhancing Substances
/ administration & dosage
Physical Conditioning, Animal
/ physiology
Proof of Concept Study
Valine
/ administration & dosage
L-Alanine
branched-chain amino acids
dietary supplements
exercise
murine model
preclinical study
resistance to fatigue
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
30 Jul 2020
30 Jul 2020
Historique:
received:
24
06
2020
revised:
23
07
2020
accepted:
27
07
2020
entrez:
6
8
2020
pubmed:
6
8
2020
medline:
16
3
2021
Statut:
epublish
Résumé
Branched-chain amino acids (BCAAs: leucine, isoleucine, valine) account for 35% of skeletal muscle essential amino acids (AAs). As such, they must be provided in the diet to support peptide synthesis and inhibit protein breakdown. Although substantial evidence has been collected about the potential usefulness of BCAAs in supporting muscle function and structure, dietary supplements containing BCAAs alone may not be effective in controlling muscle protein turnover, due to the rate-limiting bioavailability of other AAs involved in BCAAs metabolism. We aimed to evaluate the in vivo/ex vivo effects of a 4-week treatment with an oral formulation containing BCAAs alone (2:1:1) on muscle function, structure, and metabolism in a murine model of physiological exercise, which was compared to three modified formulations combining BCAAs with increasing concentrations of L-Alanine (ALA), an AA controlling BCAAs catabolism. A preliminary pharmacokinetic study confirmed the ability of ALA to boost up BCAAs bioavailability. After 4 weeks, Our study corroborates the use of BCAAs + ALA to support muscle health during physiological exercise, underlining how the relative BCAAs/ALA ratio is important to control BCAAs distribution.
Sections du résumé
BACKGROUND
BACKGROUND
Branched-chain amino acids (BCAAs: leucine, isoleucine, valine) account for 35% of skeletal muscle essential amino acids (AAs). As such, they must be provided in the diet to support peptide synthesis and inhibit protein breakdown. Although substantial evidence has been collected about the potential usefulness of BCAAs in supporting muscle function and structure, dietary supplements containing BCAAs alone may not be effective in controlling muscle protein turnover, due to the rate-limiting bioavailability of other AAs involved in BCAAs metabolism.
METHODS
METHODS
We aimed to evaluate the in vivo/ex vivo effects of a 4-week treatment with an oral formulation containing BCAAs alone (2:1:1) on muscle function, structure, and metabolism in a murine model of physiological exercise, which was compared to three modified formulations combining BCAAs with increasing concentrations of L-Alanine (ALA), an AA controlling BCAAs catabolism.
RESULTS
RESULTS
A preliminary pharmacokinetic study confirmed the ability of ALA to boost up BCAAs bioavailability. After 4 weeks,
CONCLUSION
CONCLUSIONS
Our study corroborates the use of BCAAs + ALA to support muscle health during physiological exercise, underlining how the relative BCAAs/ALA ratio is important to control BCAAs distribution.
Identifiants
pubmed: 32751732
pii: nu12082295
doi: 10.3390/nu12082295
pmc: PMC7468919
pii:
doi:
Substances chimiques
Amino Acids, Branched-Chain
0
Muscle Proteins
0
Performance-Enhancing Substances
0
Isoleucine
04Y7590D77
Leucine
GMW67QNF9C
Valine
HG18B9YRS7
Alanine
OF5P57N2ZX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dompé farmaceutici S.p.A.
ID : Fondo per la Crescita Sostenibile - Bando "HORIZON 2020" PON I&C 2014-2020 (FARMIDIAB)
Organisme : PRIN.MIUR
ID : Prot. 2017FJSM9S
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