Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry.
Animals
Animals, Genetically Modified
Bacterial Proteins
/ genetics
Cell Cycle Proteins
/ genetics
Centrioles
/ metabolism
Centrosome
/ metabolism
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ cytology
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
Genes, Reporter
Green Fluorescent Proteins
/ genetics
Interphase
Luminescent Proteins
/ genetics
Mitosis
Nerve Tissue Proteins
/ genetics
Neural Stem Cells
/ cytology
Optogenetics
/ methods
Phosphorylation
Protein Serine-Threonine Kinases
/ genetics
Signal Transduction
Red Fluorescent Protein
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
27
02
2020
accepted:
13
07
2020
revised:
18
08
2020
pubmed:
8
8
2020
medline:
18
9
2020
entrez:
8
8
2020
Statut:
epublish
Résumé
Centrosomes, the main microtubule organizing centers (MTOCs) of metazoan cells, contain an older "mother" and a younger "daughter" centriole. Stem cells either inherit the mother or daughter-centriole-containing centrosome, providing a possible mechanism for biased delivery of cell fate determinants. However, the mechanisms regulating centrosome asymmetry and biased centrosome segregation are unclear. Using 3D-structured illumination microscopy (3D-SIM) and live-cell imaging, we show in fly neural stem cells (neuroblasts) that the mitotic kinase Polo and its centriolar protein substrate Centrobin (Cnb) accumulate on the daughter centriole during mitosis, thereby generating molecularly distinct mother and daughter centrioles before interphase. Cnb's asymmetric localization, potentially involving a direct relocalization mechanism, is regulated by Polo-mediated phosphorylation, whereas Polo's daughter centriole enrichment requires both Wdr62 and Cnb. Based on optogenetic protein mislocalization experiments, we propose that the establishment of centriole asymmetry in mitosis primes biased interphase MTOC activity, necessary for correct spindle orientation.
Identifiants
pubmed: 32760088
doi: 10.1371/journal.pbio.3000762
pii: PBIOLOGY-D-20-00511
pmc: PMC7433902
doi:
Substances chimiques
Bacterial Proteins
0
Cell Cycle Proteins
0
Cnb protein, Drosophila
0
Drosophila Proteins
0
Luminescent Proteins
0
Nerve Tissue Proteins
0
Wdr62 protein, Drosophila
0
enhanced green fluorescent protein
0
yellow fluorescent protein, Bacteria
0
Green Fluorescent Proteins
147336-22-9
polo protein, Drosophila
EC 2.7.11.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000762Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM126029
Pays : United States
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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