Antioxidant, anti-inflammatory and anti-apoptotic effects of hydro-ethanolic extract of Cyperus esculentus L. (tigernut) on lead acetate-induced testicular dysfunction in Wistar rats.
Animals
Anti-Inflammatory Agents
/ isolation & purification
Antioxidants
/ isolation & purification
Apoptosis
/ drug effects
Apoptosis Regulatory Proteins
/ metabolism
Cyperus
/ chemistry
Cytoprotection
Disease Models, Animal
Ethanol
/ chemistry
Follicle Stimulating Hormone
/ blood
Inflammation Mediators
/ metabolism
Leydig Cells
/ drug effects
Lipid Peroxidation
/ drug effects
Luteinizing Hormone
/ blood
Male
Organometallic Compounds
Oxidative Stress
/ drug effects
Plant Extracts
/ isolation & purification
Rats, Wistar
Signal Transduction
Solvents
/ chemistry
Spermatozoa
/ drug effects
Testicular Diseases
/ chemically induced
Testis
/ drug effects
Testosterone
/ blood
Apoptosis
Cyperus esculentus
Lead acetate
Oxidative stress
Spermatogenesis
Steroidogenesis
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
12
05
2020
revised:
22
06
2020
accepted:
30
06
2020
pubmed:
10
8
2020
medline:
27
2
2021
entrez:
10
8
2020
Statut:
ppublish
Résumé
Lead acetate impairs testicular function by enhancing testicular oxidative stress and apoptosis. Cyperus esculentus possesses antioxidants and has shown great improvement of testicular function. This study investigated the protective effect of hydro-ethanolic extract of Cyperus esculentus on lead acetate-induced testicular dysfunction in Wistar rats. Twenty-five male Wistar rats (180-195 g) were randomly divided into 5 groups (n = 5) namely: Normal control (NC), Lead control (PbC) (20 mg/kg b.w. i.p.), C. esculentus-treated (CE) (500 mg/kg b.w p.o.), Pb + CE(500) (20 mg/kg of lead and 500 mg/kg of extract) and Pb + CE(1000) (20 mg/kg of lead and 1000 mg/kg of extract). Administration lasted for 21 days. Sperm count, motility, viability, serum testosterone and follicle stimulating hormone, Johnsen's score, Leydig cell count, Sertoli cell count, testicular testosterone, B-cell lymphoma protein-2 (Bcl-2), steroidogenic acute regulatory protein, cytochrome P450 A1, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, enzymatic antioxidant activities and total antioxidant capacity were significantly (p < 0.05) decreased in PbC compared with NC. These parameters however increased significantly (p < 0.05) in Pb + CE(500) and Pb + CE(1000) compared with PbC. Lead acetate upregulated (p < 0.05) testicular malondialdehyde, nitric oxide, glucose, lactate, lactate dehydrogenase, C-reactive protein, tumor necrosis factor-α, interleukin (IL)-6, IL-1β, Bcl-2 associated X (Bax), Bax/Bcl-2 and cleaved caspase-3 levels. All these parameters were downregulated (p < 0.05) in Pb + CE(500) and Pb + CE(1000) in comparison with PbC. C. esculentus exhibited a dose-dependent mitigation of lead acetate-induced testicular dysfunction in Wistar rats via its antioxidant, anti-inflammatory and anti-apoptotic effects.
Identifiants
pubmed: 32768970
pii: S0753-3322(20)30684-3
doi: 10.1016/j.biopha.2020.110491
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Apoptosis Regulatory Proteins
0
Inflammation Mediators
0
Organometallic Compounds
0
Plant Extracts
0
Solvents
0
Ethanol
3K9958V90M
Testosterone
3XMK78S47O
Luteinizing Hormone
9002-67-9
Follicle Stimulating Hormone
9002-68-0
lead acetate
RX077P88RY
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110491Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.