Sexually Dimorphic Crosstalk at the Maternal-Fetal Interface.
first trimester placenta
human pregnancy
placenta cell types
receptor-ligand
sex differences
single-cell RNA sequencing
Journal
The Journal of clinical endocrinology and metabolism
ISSN: 1945-7197
Titre abrégé: J Clin Endocrinol Metab
Pays: United States
ID NLM: 0375362
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
20
11
2019
accepted:
04
08
2020
pubmed:
11
8
2020
medline:
17
2
2021
entrez:
11
8
2020
Statut:
ppublish
Résumé
Crosstalk through receptor ligand interactions at the maternal-fetal interface is impacted by fetal sex. This affects placentation in the first trimester and differences in outcomes. Sexually dimorphic signaling at early stages of placentation are not defined. Investigate the impact of fetal sex on maternal-fetal crosstalk. Receptors/ligands at the maternal-fetal surface were identified from sexually dimorphic genes between fetal sexes in the first trimester placenta and defined in each cell type using single-cell RNA-Sequencing (scRNA-Seq). Academic institution. Late first trimester (~10-13 weeks) placenta (fetal) and decidua (maternal) from uncomplicated ongoing pregnancies. Transcriptomic profiling at tissue and single-cell level; immunohistochemistry of select proteins. We identified 91 sexually dimorphic receptor-ligand pairs across the maternal-fetal interface. We examined fetal sex differences in 5 major cell types (trophoblasts, stromal cells, Hofbauer cells, antigen-presenting cells, and endothelial cells). Ligands from the CC family chemokine ligand (CCL) family were most highly representative in females, with their receptors present on the maternal surface. Sexually dimorphic trophoblast transcripts, Mucin-15 (MUC15) and notum, palmitoleoyl-protein carboxylesterase (NOTUM) were also most highly expressed in syncytiotrophoblasts and extra-villous trophoblasts respectively. Gene Ontology (GO) analysis using sexually dimorphic genes in individual cell types identified cytokine mediated signaling pathways to be most representative in female trophoblasts. Upstream analysis demonstrated TGFB1 and estradiol to affect all cell types, but dihydrotestosterone, produced by the male fetus, was an upstream regulator most significant for the trophoblast population. Maternal-fetal crosstalk exhibits sexual dimorphism during placentation early in gestation.
Identifiants
pubmed: 32772088
pii: 5890142
doi: 10.1210/clinem/dgaa503
pmc: PMC7571453
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NICHD NIH HHS
ID : R01 HD074368
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD091773
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007770
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
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