Growth of Giant Peptide Vesicles Driven by Compartmentalized Transcription-Translation Activity.
artificial cells
elastin-like polypeptides
membranes
synthetic biology
vesicle growth
Journal
Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783
Informations de publication
Date de publication:
23 Dec 2020
23 Dec 2020
Historique:
received:
23
07
2020
pubmed:
11
8
2020
medline:
23
2
2021
entrez:
11
8
2020
Statut:
ppublish
Résumé
Compartmentalization and spatial organization of biochemical reactions are essential for the establishment of complex metabolic pathways inside synthetic cells. Phospholipid and fatty acid membranes are the most natural candidates for this purpose, but also polymers have shown great potential as enclosures of artificial cell mimics. Herein, we report on the formation of giant vesicles in a size range of 1 μm-100 μm using amphiphilic elastin-like polypeptides. The peptide vesicles can accommodate cell-free gene expression reactions, which is demonstrated by the transcription of a fluorescent RNA aptamer and the production of a fluorescent protein. Importantly, gene expression inside the vesicles leads to a strong growth of their size-up to an order of magnitude in volume in several cases-which is driven by changes in osmotic pressure, resulting in fusion events and uptake of membrane peptides from the environment.
Identifiants
pubmed: 32777105
doi: 10.1002/chem.202003366
pmc: PMC7839564
doi:
Substances chimiques
Peptides
0
Phospholipids
0
Polymers
0
Elastin
9007-58-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
17356-17360Subventions
Organisme : Hanns-Seidel-Stiftung
Organisme : Deutsche Forschungsgemeinschaft
ID : Project-ID 364653263 - TRR 235/P15
Organisme : Deutsche Forschungsgemeinschaft
ID : RTG 2062
Organisme : FP7 Ideas: European Research Council
ID : 694410
Informations de copyright
© 2020 The Authors. Published by Wiley-VCH GmbH.
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