Purification and characterization of an inulinase produced by a Kluyveromyces marxianus strain isolated from blue agave bagasse.
Branched inulin
Enzymatic hydrolysis
Inulinase
Kluyveromyces marxianus
Journal
Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
10
04
2020
revised:
25
07
2020
accepted:
25
07
2020
pubmed:
11
8
2020
medline:
29
1
2021
entrez:
11
8
2020
Statut:
ppublish
Résumé
Exo-inulinases are versatile enzymes that have gained attention in recent years due to their ability to hydrolyze linear and branched polyfructose chains found in inulines. Agavin, a branched inulin, is found in Agave plant, the raw matter to produce tequila. Our group has isolated several microbial strains from agave bagasse, an agro-industrial residue from tequila production that increases yearly. Strain ISO3, identified as Kluyveromyces marxianus, showed a remarkable activity towards agavin, and from its fermentation liquor an inulinolytic enzyme (Inu-ISO3) was purified. The isolated enzyme is a glycosylated dimeric protein with a molecular mass of ~256 kDa, as determined by DLS and SEC. The enzyme has an isoelectric pH of 4.6 and has both inulinase and invertase activities with an I/S ratio (ratio of activity with agavin to activity with sucrose) of 1.39. The enzyme has temperature and pH optima of 50 °C and 5.5, respectively, and follows hyperbolic kinetics with agavin (k
Identifiants
pubmed: 32777449
pii: S1046-5928(20)30309-0
doi: 10.1016/j.pep.2020.105718
pii:
doi:
Substances chimiques
Fungal Proteins
0
Inulin
9005-80-5
Glycoside Hydrolases
EC 3.2.1.-
inulinase
EC 3.2.1.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105718Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.