An effector essential for virulence of necrotrophic fungi targets plant HIRs to inhibit host immunity.
Arabidopsis
/ immunology
Ascomycota
/ pathogenicity
Plant Diseases
/ microbiology
Plants, Genetically Modified
Plant Immunity
/ genetics
Arabidopsis Proteins
/ genetics
Virulence
/ genetics
Reactive Oxygen Species
/ metabolism
Fungal Proteins
/ metabolism
Gene Expression Regulation, Plant
Host-Pathogen Interactions
/ immunology
Salicylic Acid
/ metabolism
Pathogen-Associated Molecular Pattern Molecules
/ metabolism
Mutation
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
30 Oct 2024
30 Oct 2024
Historique:
received:
10
10
2023
accepted:
20
10
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
Phytopathogens often secrete effectors to enhance their infection of plants. In the case of Sclerotinia sclerotiorum, a necrotrophic phytopathogen, a secreted protein named SsPEIE1 (Sclerotinia sclerotiorum Plant Early Immunosuppressive Effector 1) plays a crucial role in its virulence. During the early stages of infection, SsPEIE1 is significantly up-regulated. Additionally, transgenic plants expressing SsPEIE1 exhibit increased susceptibility to different phytopathogens. Further investigations revealed that SsPEIE1 interacts with a plasma membrane protein known as hypersensitive induced reaction (HIR) that dampens immune responses. SsPEIE1 is required for S. sclerotiorum virulence on wild-type Arabidopsis but not on Arabidopsis hir4 mutants. Moreover, Arabidopsis hir2 and hir4 mutants exhibit suppressed pathogen-associated molecular pattern-triggered reactive oxygen species (ROS) bursts and salicylic acid (SA)-associated immune gene induction, all of which are phenocopied by the SsPEIE1 transgenic plants. We find that the oligomerization of AtHIR4 is essential for its role in mediating immunity, and that SsPEIE1 inhibits its oligomerization through competitively binding to AtHIR4. Remarkably, both Arabidopsis and rapeseed plants overexpress AtHIR4 display significantly increased resistance to S. sclerotiorum. In summary, these results demonstrate that SsPEIE1 inhibits AtHIR4 oligomerization-mediated immune responses by interacting with the key immune factor AtHIR4, thereby promoting S. sclerotiorum infection.
Identifiants
pubmed: 39477937
doi: 10.1038/s41467-024-53725-0
pii: 10.1038/s41467-024-53725-0
doi:
Substances chimiques
Arabidopsis Proteins
0
Reactive Oxygen Species
0
Fungal Proteins
0
Salicylic Acid
O414PZ4LPZ
Pathogen-Associated Molecular Pattern Molecules
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9391Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 32172368, 32130087
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 3213008
Organisme : Huazhong Agricultural University (HZAU)
ID : 2021ZKPY014
Informations de copyright
© 2024. The Author(s).
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