Decoding the Functional Evolution of an Intramembrane Protease Superfamily by Statistical Coupling Analysis.
Ras-converting enzyme-1
gamma-secretase
membrane protein evolution
presenilin
proteolysis
site-2 protease
statistical coupling analysis
Journal
Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
04
05
2020
revised:
12
06
2020
accepted:
24
07
2020
pubmed:
17
8
2020
medline:
5
10
2021
entrez:
16
8
2020
Statut:
ppublish
Résumé
How evolution endowed membrane enzymes with specific abilities, and then tuned them to the needs of different cells, is poorly understood. We examined whether statistical coupling analysis (SCA) can be applied to rhomboid proteases, the most widely distributed membrane proteins, to identify amino acid "sectors" that evolved independently to acquire a specific function. SCA revealed three coevolving residue networks that form two sectors. Sector 1 determines substrate specificity, but is paradoxically scattered across the protein, consistent with dynamics driving rhomboid-substrate interactions. Sector 2 is hierarchically composed of a subgroup that maintains the catalytic site, and another that maintains the overall fold, forecasting evolution of rhomboid pseudoproteases. Changing only sector 1 residues of a "recipient" rhomboid converted its substrate specificity and catalytic efficiency to that of the "donor." While used only twice over a decade ago, SCA should be generally applicable to membrane proteins, and our sector grafting approach provides an efficient strategy for designing enzymes.
Identifiants
pubmed: 32795403
pii: S0969-2126(20)30279-3
doi: 10.1016/j.str.2020.07.015
pmc: PMC7710553
mid: NIHMS1621350
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Escherichia coli Proteins
0
GlpG protein, E coli
0
Membrane Proteins
0
Endopeptidases
EC 3.4.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1329-1336.e4Subventions
Organisme : NIAID NIH HHS
ID : R01 AI066025
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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