Peroxisome retention involves Inp1-dependent peroxisome-plasma membrane contact sites in yeast.
Actin Cytoskeleton
/ genetics
Bridged Bicyclo Compounds, Heterocyclic
/ pharmacology
Cell Membrane
/ genetics
Endoplasmic Reticulum
/ genetics
Gene Expression Regulation, Fungal
/ genetics
Membrane Proteins
/ genetics
Mitochondrial Membranes
/ drug effects
Peroxins
/ genetics
Peroxisomes
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Saccharomycetales
/ genetics
Thiazolidines
/ pharmacology
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
05 10 2020
05 10 2020
Historique:
received:
04
06
2019
revised:
15
03
2020
accepted:
23
06
2020
entrez:
18
8
2020
pubmed:
18
8
2020
medline:
25
3
2021
Statut:
ppublish
Résumé
Retention of peroxisomes in yeast mother cells requires Inp1, which is recruited to the organelle by the peroxisomal membrane protein Pex3. Here we show that Hansenula polymorpha Inp1 associates peroxisomes to the plasma membrane. Peroxisome-plasma membrane contact sites disappear upon deletion of INP1 but increase upon INP1 overexpression. Analysis of truncated Inp1 variants showed that the C terminus is important for association to the peroxisome, while a stretch of conserved positive charges and a central pleckstrin homology-like domain are important for plasma membrane binding. In cells of a PEX3 deletion, strain Inp1-GFP localizes to the plasma membrane, concentrated in patches near the bud neck and in the cortex of nascent buds. Upon disruption of the actin cytoskeleton by treatment of the cells with latrunculin A, Inp1-GFP became cytosolic, indicating that Inp1 localization is dependent on the presence of an intact actin cytoskeleton.
Identifiants
pubmed: 32805027
pii: 152028
doi: 10.1083/jcb.201906023
pmc: PMC7659721
pii:
doi:
Substances chimiques
Bridged Bicyclo Compounds, Heterocyclic
0
INP1 protein, S cerevisiae
0
Membrane Proteins
0
PEX3 protein, S cerevisiae
0
Peroxins
0
Saccharomyces cerevisiae Proteins
0
Thiazolidines
0
latrunculin A
SRQ9WWM084
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M011801/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P003818/1
Pays : United Kingdom
Informations de copyright
© 2020 Krikken et al.
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