Five-year cryopreservation at -80 °C of edible and medicinal basidiomycetes by wheat grain technique.
Agaricus
Basidiomycete
Cryoprotectant
Long-term cryopreservation
Mushroom
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
15
07
2020
revised:
06
08
2020
accepted:
10
08
2020
pubmed:
18
8
2020
medline:
23
7
2021
entrez:
18
8
2020
Statut:
ppublish
Résumé
This research has focused on basidiomycete cryopreservation at -80 °C and developed a cryopreservation method based on the use of hard or medium-hard endosperm wheat grains as a mycelial carrier for cryopreservation. The aim of this study was to evaluate the mycelial viability of edible and medicinal basidiomycetes, using 13 strains of Agaricus spp. and eight strains of non-Agaricus spp., cryopreserved at -80 °C on hard endosperm wheat grain, with or without cryoprotectant agent (4% glucose), for two and five years. Two groups of basidiomycetes, Agaricus genus and other non-Agaricus genera, were cryopreserved at -80 °C by wheat grain technique for two and five years. The cryopreservation technique with hard endosperm wheat grain without cryoprotectant (preservation substrate), settled previously for A. subrufescens is efficient to cryopreserve other basidiomycetes such as Lentinus crinitus, Pleurotus ostreatus, Pleurotus eryngii, Schizophyllum commune, and Lentinula edodes, besides A. subrufescens strains.
Identifiants
pubmed: 32805366
pii: S0167-7012(20)30746-6
doi: 10.1016/j.mimet.2020.106030
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
106030Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no competing interests.