Targeting the honey bee gut parasite Nosema ceranae with siRNA positively affects gut bacteria.
Animals
Bacteria
/ classification
Bees
/ microbiology
DNA, Bacterial
/ genetics
Gastrointestinal Microbiome
/ drug effects
High-Throughput Nucleotide Sequencing
Microsporidiosis
/ prevention & control
Nosema
/ drug effects
Phylogeny
RNA, Small Interfering
/ pharmacology
Sequence Analysis, DNA
/ methods
Bacteria
Honey bee
Metatranscriptomics
Nosema ceranae
siRNA
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
17 08 2020
17 08 2020
Historique:
received:
22
08
2019
accepted:
10
08
2020
entrez:
19
8
2020
pubmed:
19
8
2020
medline:
13
7
2021
Statut:
epublish
Résumé
Gut microbial communities can contribute positively and negatively to host health. So far, eight core bacterial taxonomic clusters have been reported in honey bees. These bacteria are involved in host metabolism and defenses. Nosema ceranae is a gut intracellular parasite of honey bees which destroys epithelial cells and gut tissue integrity. Studies have shown protective impacts of honey bee gut microbiota towards N. ceranae infection. However, the impacts of N. ceranae on the relative abundance of honey bee gut microbiota remains unclear, and has been confounded during prior infection assays which resulted in the co-inoculation of bacteria during Nosema challenges. We used a novel method, the suppression of N. ceranae with specific siRNAs, to measure the impacts of Nosema on the gut microbiome. Suppressing N. ceranae led to significant positive effects on microbial abundance. Nevertheless, 15 bacterial taxa, including three core taxa, were negatively correlated with N. ceranae levels. In particular, one co-regulated group of 7 bacteria was significantly negatively correlated with N. ceranae levels. N. ceranae are negatively correlated with the abundance of 15 identified bacteria. Our results provide insights into interactions between gut microbes and N. ceranae during infection.
Sections du résumé
BACKGROUND
Gut microbial communities can contribute positively and negatively to host health. So far, eight core bacterial taxonomic clusters have been reported in honey bees. These bacteria are involved in host metabolism and defenses. Nosema ceranae is a gut intracellular parasite of honey bees which destroys epithelial cells and gut tissue integrity. Studies have shown protective impacts of honey bee gut microbiota towards N. ceranae infection. However, the impacts of N. ceranae on the relative abundance of honey bee gut microbiota remains unclear, and has been confounded during prior infection assays which resulted in the co-inoculation of bacteria during Nosema challenges. We used a novel method, the suppression of N. ceranae with specific siRNAs, to measure the impacts of Nosema on the gut microbiome.
RESULTS
Suppressing N. ceranae led to significant positive effects on microbial abundance. Nevertheless, 15 bacterial taxa, including three core taxa, were negatively correlated with N. ceranae levels. In particular, one co-regulated group of 7 bacteria was significantly negatively correlated with N. ceranae levels.
CONCLUSIONS
N. ceranae are negatively correlated with the abundance of 15 identified bacteria. Our results provide insights into interactions between gut microbes and N. ceranae during infection.
Identifiants
pubmed: 32807095
doi: 10.1186/s12866-020-01939-9
pii: 10.1186/s12866-020-01939-9
pmc: PMC7433167
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
258Subventions
Organisme : Jiangxi Agricultural University
ID : 050014/923230722
Pays : International
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