Structural analysis and reaction mechanism of the disproportionating enzyme (D-enzyme) from potato.

Glycogen Debranching Enzyme System / chemistry Plant Proteins / chemistry Protein Domains Solanum tuberosum / enzymology Starch / chemistry
4-α-glucanotransferase D-enzyme acarbose crystal structure cycloamylose potato

Journal

Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750

Informations de publication

Date de publication:
10 2020
Historique:
received: 03 07 2020
revised: 14 08 2020
accepted: 14 08 2020
pubmed: 19 8 2020
medline: 22 1 2021
entrez: 19 8 2020
Statut: ppublish

Résumé

Starch produced by plants is a stored form of energy and is an important dietary source of calories for humans and domestic animals. Disproportionating enzyme (D-enzyme) catalyzes intramolecular and intermolecular transglycosylation reactions of α-1, 4-glucan. D-enzyme is essential in starch metabolism in the potato. We present the crystal structures of potato D-enzyme, including two different types of complex structures: a primary Michaelis complex (substrate binding mode) for 26-meric cycloamylose (CA26) and a covalent intermediate for acarbose. Our study revealed that the acarbose and CA26 reactions catalyzed by potato D-enzyme involve the formation of a covalent intermediate with the donor substrate. HPAEC of reaction substrates and products revealed the activity of the potato D-enzyme on acarbose and CA26 as donor substrates. The structural and chromatography analyses provide insight into the mechanism of the coupling reaction of CA and glucose catalyzed by the potato D-enzyme. The enzymatic reaction mechanism does not involve residual hydrolysis. This could be particularly useful in preventing unnecessary starch degradation leading to reduced crop productivity. Optimization of this mechanism would be important for improvements of starch storage and productivity in crops.

Identifiants

DOI: 10.1002/pro.3932 PMID: 32808707 PMC: PMC7513719
pubmed: 32808707
doi: 10.1002/pro.3932
pmc: PMC7513719
doi:

Substances chimiques

Glycogen Debranching Enzyme System 0
Plant Proteins 0
Starch 9005-25-8
4 alpha-glucanotransferase EC 2.4.1.25

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2085-2100

Informations de copyright

© 2020 The Protein Society.

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Auteurs

Kayo Imamura (K)

Laboratory of Enzyme Chemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Osaka, Japan.

Takanori Matsuura (T)

Institute for Protein Research, Osaka University, Osaka, Japan.

Atsushi Nakagawa (A)

Institute for Protein Research, Osaka University, Osaka, Japan.

Shinichi Kitamura (S)

Laboratory of Biophysical Chemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Osaka, Japan.

Masami Kusunoki (M)

Institute for Protein Research, Osaka University, Osaka, Japan.

Takeshi Takaha (T)

Biochemical Research Laboratories, Ezaki Glico Co., Ltd, Osaka, Japan.

Hideaki Unno (H)

Graduate School of Engineering, Nagasaki University, Nagasaki, Japan.
Organization for Marine Science and Technology, Nagasaki University, Nagasaki, Japan.
ORCID: 0000-0001-5917-269X

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Glycosyltransferase › Hexosyltransferases › Glucosyltransferases › Glycogen debranching enzyme system › Structural analysis and reaction mechanism of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Structural analysis and reaction mechanism of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Structural analysis and reaction mechanism of...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Solanales › Solanaceae › Solanum › Solanum tuberosum › Structural analysis and reaction mechanism of...
questionsmedicales.fr › Glucides › Polyosides › Glucanes › Amidon › Structural analysis and reaction mechanism of...