High titer methyl ketone production with tailored Pseudomonas taiwanensis VLB120.

Acetone Fermentation Metabolic Engineering Pseudomonas / genetics
Biodiesel Metabolic engineering Metabolic modeling Pseudomonads Synthetic biology Thioesterase

Journal

Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657

Informations de publication

Date de publication:
11 2020
Historique:
received: 29 05 2020
revised: 13 07 2020
accepted: 05 08 2020
pubmed: 19 8 2020
medline: 27 10 2021
entrez: 19 8 2020
Statut: ppublish

Résumé

Methyl ketones present a group of highly reduced platform chemicals industrially produced from petroleum-derived hydrocarbons. They find applications in the fragrance, flavor, pharmacological, and agrochemical industries, and are further discussed as biodiesel blends. In recent years, intense research has been carried out to achieve sustainable production of these molecules by re-arranging the fatty acid metabolism of various microbes. One challenge in the development of a highly productive microbe is the high demand for reducing power. Here, we engineered Pseudomonas taiwanensis VLB120 for methyl ketone production as this microbe has been shown to sustain exceptionally high NAD(P)H regeneration rates. The implementation of published strategies resulted in 2.1 g L

Identifiants

DOI: 10.1016/j.ymben.2020.08.003 PMID: 32810591
pubmed: 32810591
pii: S1096-7176(20)30122-1
doi: 10.1016/j.ymben.2020.08.003
pii:
doi:

Substances chimiques

Acetone 1364PS73AF

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

84-94

Informations de copyright

Copyright © 2020 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Auteurs

Salome C Nies (SC)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

Tobias B Alter (TB)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

Sophia Nölting (S)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

Susanne Thiery (S)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

An N T Phan (ANT)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

Noud Drummen (N)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

Jay D Keasling (JD)

Joint BioEnergy Institute, Emeryville, CA, 94608, USA; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800, Lyngby, Denmark; Lawrence Berkeley National Laboratory, Biological Systems and Engineering Division, Berkeley, CA, 94720, USA; Virtual Institute of Microbial Stress and Survival, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA; Dept. of Bioengineering, University of California, Berkeley, CA, 94720, USA; Dept. of Chemical Engineering, University of California, Berkeley, CA, 94720, USA; Synthetic Biochemistry Center, Institute for Synthetic Biology, Shenzhen Institutes for Advanced Technologies, Shenzhen, China.

Lars M Blank (LM)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany.

Birgitta E Ebert (BE)

iAMB-Institute of Applied Microbiology, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, DE, Germany; Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD, 4072, Australia; CSIRO Future Science Platform in Synthetic Biology, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Black Mountain, ACT, 2601, Australia. Electronic address: birgitta.ebert@uq.edu.au.

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Cétones › Acétone › High titer methyl ketone production with...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › High titer methyl ketone production with...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › High titer methyl ketone production with...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › High titer methyl ketone production with...