HIV-1 Lethality and Loss of Env Protein Expression Induced by Single Synonymous Substitutions in the Virus Genome Intronic-Splicing Silencer.
Base Pairing
Base Sequence
CD4-Positive T-Lymphocytes
/ metabolism
Cell Line
Codon
Exons
Genome, Viral
HEK293 Cells
HIV-1
/ genetics
Humans
Introns
RNA Folding
RNA Splicing
Silent Mutation
Structure-Activity Relationship
Thermodynamics
Virus Replication
/ genetics
env Gene Products, Human Immunodeficiency Virus
/ chemistry
envelope
human immunodeficiency virus
lethality
synonymous substitutions
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
14 10 2020
14 10 2020
Historique:
received:
02
06
2020
accepted:
06
08
2020
pubmed:
21
8
2020
medline:
31
12
2020
entrez:
21
8
2020
Statut:
epublish
Résumé
Synonymous genome recoding has been widely used to study different aspects of virus biology. Codon usage affects the temporal regulation of viral gene expression. In this study, we performed synonymous codon mutagenesis to investigate whether codon usage affected HIV-1 Env protein expression and virus viability. We replaced the codons AGG, GAG, CCU, ACU, CUC, and GGG of the HIV-1
Identifiants
pubmed: 32817222
pii: JVI.01108-20
doi: 10.1128/JVI.01108-20
pmc: PMC7565636
pii:
doi:
Substances chimiques
Codon
0
env Gene Products, Human Immunodeficiency Virus
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 American Society for Microbiology.
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