A β-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL.
Bacterial Outer Membrane Proteins
/ chemistry
Bacterial Proteins
/ metabolism
Hydrophobic and Hydrophilic Interactions
Lipid Bilayers
/ chemistry
Magnetic Resonance Imaging
/ methods
Magnetic Resonance Spectroscopy
/ methods
Membrane Lipids
/ chemistry
Membrane Proteins
/ metabolism
Molecular Dynamics Simulation
Nuclear Magnetic Resonance, Biomolecular
/ methods
Permeability
Protein Structure, Secondary
lipid bilayers
magic-angle spinning
membrane protein
protein dynamics
protein structure
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
pubmed:
21
8
2020
medline:
30
10
2020
entrez:
21
8
2020
Statut:
ppublish
Résumé
The protein AlkL is known to increase permeability of the outer membrane of bacteria for hydrophobic molecules, yet the mechanism of transport has not been determined. Differing crystal and NMR structures of homologous proteins resulted in a controversy regarding the degree of structure and the role of long extracellular loops. Here we solve this controversy by determining the de novo NMR structure in near-native lipid bilayers, and by accessing structural dynamics relevant to hydrophobic substrate permeation through molecular-dynamics simulations and by characteristic NMR relaxation parameters. Dynamic lateral exit sites large enough to accommodate substrates such as carvone or octane occur through restructuring of a barrel extension formed by the extracellular loops.
Identifiants
pubmed: 32817429
pii: 2002598117
doi: 10.1073/pnas.2002598117
pmc: PMC7474606
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
Bacterial Proteins
0
Lipid Bilayers
0
Membrane Lipids
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21014-21021Informations de copyright
Copyright © 2020 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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