LIVE-PAINT allows super-resolution microscopy inside living cells using reversible peptide-protein interactions.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
20 08 2020
20 08 2020
Historique:
received:
19
02
2020
accepted:
30
07
2020
entrez:
22
8
2020
pubmed:
21
8
2020
medline:
23
6
2021
Statut:
epublish
Résumé
We present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied, unlike conventional super-resolution methods, which rely on directly fusing the biomolecule of interest to a large fluorescent protein, organic fluorophore, or oligonucleotide. LIVE-PAINT works by observing the blinking of localized fluorescence as this peptide is reversibly bound by a protein that is fused to a fluorescent protein. We have demonstrated the effectiveness of LIVE-PAINT by imaging a number of different proteins inside live S. cerevisiae. Not only is LIVE-PAINT widely applicable, easily implemented, and the modifications minimally perturbing, but we also anticipate it will extend data acquisition times compared to those previously possible with methods that involve direct fusion to a fluorescent protein.
Identifiants
pubmed: 32820217
doi: 10.1038/s42003-020-01188-6
pii: 10.1038/s42003-020-01188-6
pmc: PMC7441314
doi:
Substances chimiques
Escherichia coli Proteins
0
Fungal Proteins
0
Peptides
0
Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
458Subventions
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : T32 GM008283
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM118528
Pays : United States
Organisme : NIGMS NIH HHS
ID : 118528
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM149438
Pays : United States
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