Characterization of KIR + NKG2A + Eomes- NK-like CD8+ T cells and their decline with age in healthy individuals.
Adolescent
Adult
Aged
Aged, 80 and over
Aging
/ blood
CD8-Positive T-Lymphocytes
/ metabolism
Child
Female
Fetal Blood
/ metabolism
Flow Cytometry
/ methods
Gene Expression Regulation
Humans
Interferon-gamma
/ blood
Killer Cells, Natural
/ metabolism
Male
Middle Aged
NK Cell Lectin-Like Receptor Subfamily C
/ blood
Receptors, KIR3DL1
/ genetics
T-Box Domain Proteins
/ blood
T-Lymphocyte Subsets
/ metabolism
Young Adult
NK-like CD8+ T cells
aging
differentiation
memory T cell
Journal
Cytometry. Part B, Clinical cytometry
ISSN: 1552-4957
Titre abrégé: Cytometry B Clin Cytom
Pays: United States
ID NLM: 101235690
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
03
07
2020
received:
03
04
2020
accepted:
21
07
2020
pubmed:
25
8
2020
medline:
8
1
2022
entrez:
25
8
2020
Statut:
ppublish
Résumé
KIR+NKG2A + Eomes+ CD8+ T cells, which are preferentially found with a T We compare the distribution of the memory phenotypes and senescence-associated markers of two T-cell subsets by multicolor flow cytometry in 10 cord blood samples and 105 healthy individuals (HIs) ranging from 6 to 84 years of age. We found that the Eomes+ population has a higher differentiation degree than the Eomes- population. T cells in the Eomes- subset show proportionally less T Overall, the KIR+NKG2A + Eomes- CD8+ T-cell population shares similar characteristics with the Eomes+ population, although with a lower degree of differentiation, lower senescence marker expression, and a proportional decrease with age. Thus, we suspect that KIR+NKG2A + Eomes-CD8+ T cells may represent a less differentiated stage of the NK-like CD8+ T-cell subset.
Sections du résumé
BACKGROUND
KIR+NKG2A + Eomes+ CD8+ T cells, which are preferentially found with a T
METHODS
We compare the distribution of the memory phenotypes and senescence-associated markers of two T-cell subsets by multicolor flow cytometry in 10 cord blood samples and 105 healthy individuals (HIs) ranging from 6 to 84 years of age.
RESULTS
We found that the Eomes+ population has a higher differentiation degree than the Eomes- population. T cells in the Eomes- subset show proportionally less T
CONCLUSION
Overall, the KIR+NKG2A + Eomes- CD8+ T-cell population shares similar characteristics with the Eomes+ population, although with a lower degree of differentiation, lower senescence marker expression, and a proportional decrease with age. Thus, we suspect that KIR+NKG2A + Eomes-CD8+ T cells may represent a less differentiated stage of the NK-like CD8+ T-cell subset.
Identifiants
pubmed: 32830898
doi: 10.1002/cyto.b.21945
doi:
Substances chimiques
EOMES protein, human
0
IFNG protein, human
0
KLRC1 protein, human
0
NK Cell Lectin-Like Receptor Subfamily C
0
Receptors, KIR3DL1
0
T-Box Domain Proteins
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
467-475Informations de copyright
© 2020 International Clinical Cytometry Society.
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