Control of ribosome synthesis in bacteria: the important role of rRNA chain elongation rate.
GFP reporter system
Vibrio natriegens
low temperature
rRNA chain elongation rate
ribosome synthesis
rrn transcriptional initiation rate
Journal
Science China. Life sciences
ISSN: 1869-1889
Titre abrégé: Sci China Life Sci
Pays: China
ID NLM: 101529880
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
09
03
2020
accepted:
26
05
2020
pubmed:
26
8
2020
medline:
28
12
2021
entrez:
26
8
2020
Statut:
ppublish
Résumé
Bacteria growth depends crucially on protein synthesis, which is limited by ribosome synthesis. Ribosomal RNA (rRNA) transcription is the rate-limiting step of ribosome synthesis. It is generally proposed that the transcriptional initiation rate of rRNA operon is the primary factor that controls the rRNA synthesis. In this study, we established a convenient GFP-based reporter approach for measuring the bacterial rRNA chain elongation rate. We showed that the rRNA chain elongation rate of Escherichia coli remains constant under nutrient limitation and chloramphenicol inhibition. In contrast, rRNA chain elongation rate decreases dramatically under low temperatures. Strikingly, we found that Vibrio natriegens, the fastest growing bacteria known, has a 50% higher rRNA chain elongation rate than E. coli, which contributes to its rapid ribosome synthesis. Our study demonstrates that rRNA chain elongation rate is another important factor that affects the bacterial ribosome synthesis capacity.
Identifiants
pubmed: 32840734
doi: 10.1007/s11427-020-1742-4
pii: 10.1007/s11427-020-1742-4
doi:
Substances chimiques
RNA, Bacterial
0
RNA, Ribosomal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
795-802Références
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