Fractionation and characterization of polyphenolic compounds and macromolecules in red wine by asymmetrical flow field-flow fractionation.
AF4
Macromolecular complexes
Polyphenols
Polysaccharides
Red wine
Tannins
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
11 Oct 2020
11 Oct 2020
Historique:
received:
30
04
2020
revised:
06
08
2020
accepted:
07
08
2020
pubmed:
26
8
2020
medline:
5
11
2020
entrez:
26
8
2020
Statut:
ppublish
Résumé
Red wine is a complex matrix containing macromolecules such as condensed tannins and polysaccharides. Wine macromolecular components and their interactions have been reported to impact taste properties such as astringency but the colloidal systems formed in wine are not well known. A key prerequisite to characterize these systems is the ability to work under analytical conditions as close as possible to the colloid environment, preserving the sample structure and limiting the denaturation of macromolecular complexes. A method of Asymmetric Flow Field-Flow Fractionation (AF4) coupled with UV detection, multi-angle light scattering (MALS), and differential refractometer index (dRI) (AF4-UV-MALS-dRI) has been developed to analyse macromolecules, including tannins and polysaccharides, and macromolecular complexes, in red wine. This method separates objects according to their hydrodynamic radius and does not require calibration to determine molecular weight (M
Identifiants
pubmed: 32841772
pii: S0021-9673(20)30740-8
doi: 10.1016/j.chroma.2020.461464
pii:
doi:
Substances chimiques
Macromolecular Substances
0
Polyphenols
0
Polysaccharides
0
Tannins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
461464Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None.