Establishment of Proximity-Dependent Biotinylation Approaches in Different Plant Model Systems.
Arabidopsis
/ cytology
Biotin
/ chemistry
Biotinylation
Carbon-Nitrogen Ligases
/ genetics
Cell Membrane
/ metabolism
Escherichia coli Proteins
/ genetics
Green Fluorescent Proteins
/ genetics
Lotus
/ genetics
Solanum lycopersicum
/ chemistry
Plant Proteins
/ chemistry
Plants, Genetically Modified
Protein Interaction Maps
Protein Subunits
Recombinant Proteins
/ chemistry
Repressor Proteins
/ genetics
Temperature
Nicotiana
/ genetics
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
25
03
2020
revised:
22
06
2020
accepted:
21
08
2020
pubmed:
28
8
2020
medline:
3
6
2021
entrez:
27
8
2020
Statut:
ppublish
Résumé
Proximity labeling is a powerful approach for detecting protein-protein interactions. Most proximity labeling techniques use a promiscuous biotin ligase or a peroxidase fused to a protein of interest, enabling the covalent biotin labeling of proteins and subsequent capture and identification of interacting and neighboring proteins without the need for the protein complex to remain intact. To date, only a few studies have reported on the use of proximity labeling in plants. Here, we present the results of a systematic study applying a variety of biotin-based proximity labeling approaches in several plant systems using various conditions and bait proteins. We show that TurboID is the most promiscuous variant in several plant model systems and establish protocols that combine mass spectrometry-based analysis with harsh extraction and washing conditions. We demonstrate the applicability of TurboID in capturing membrane-associated protein interactomes using
Identifiants
pubmed: 32843435
pii: 6099389
doi: 10.1105/tpc.20.00235
pmc: PMC7610282
doi:
Substances chimiques
Escherichia coli Proteins
0
Plant Proteins
0
Protein Subunits
0
Recombinant Proteins
0
Repressor Proteins
0
Green Fluorescent Proteins
147336-22-9
Biotin
6SO6U10H04
Carbon-Nitrogen Ligases
EC 6.3.-
birA protein, E coli
EC 6.3.4.15
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3388-3407Subventions
Organisme : European Research Council
ID : 682436
Pays : International
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2020 American Society of Plant Biologists. All rights reserved.
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