Improved reference genome of the arboviral vector Aedes albopictus.
Ae. albopictus
Developmental transcriptome
Genome
Immunity
Population differentiation
Sex locus
Viral integrations
miRNAs
piRNA clusters
Journal
Genome biology
ISSN: 1474-760X
Titre abrégé: Genome Biol
Pays: England
ID NLM: 100960660
Informations de publication
Date de publication:
26 08 2020
26 08 2020
Historique:
received:
26
04
2020
accepted:
07
08
2020
entrez:
28
8
2020
pubmed:
28
8
2020
medline:
13
7
2021
Statut:
epublish
Résumé
The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes. We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes. The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.
Sections du résumé
BACKGROUND
The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes.
RESULTS
We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes.
CONCLUSION
The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.
Identifiants
pubmed: 32847630
doi: 10.1186/s13059-020-02141-w
pii: 10.1186/s13059-020-02141-w
pmc: PMC7448346
doi:
Substances chimiques
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
215Subventions
Organisme : NIAID NIH HHS
ID : R01 AI151004
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI135258
Pays : United States
Organisme : Foundation for the National Institutes of Health
ID : 1R01AI151004-01
Pays : International
Organisme : Foundation for the National Institutes of Health
ID : R01AI32409
Pays : International
Organisme : Foundation for the National Institutes of Health
ID : 1DP2AI152071-01
Pays : International
Organisme : Foundation for the National Institutes of Health
ID : R21AI135258
Pays : International
Organisme : NIAID NIH HHS
ID : DP2 AI152071
Pays : United States
Commentaires et corrections
Type : ErratumIn
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