Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution.
Staphylococcus aureus
antibiotic resistance
drug targets
methyltransferases
rRNA modifications
ribosome
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
10
07
2020
revised:
11
08
2020
accepted:
17
08
2020
pubmed:
28
8
2020
medline:
21
5
2021
entrez:
28
8
2020
Statut:
ppublish
Résumé
Staphylococcus aureus is a bacterial pathogen and one of the leading causes of healthcare-acquired infections in the world. The growing antibiotic resistance of S. aureus obliges us to search for new drugs and treatments. As the majority of antibiotics target the ribosome, knowledge of its detailed structure is crucial for drug development. Here, we report the cryo-EM reconstruction at 3.2 Å resolution of the S. aureus ribosome with P-site tRNA, messenger RNA, and 10 RNA modification sites previously not assigned or visualized. The resulting model is the most precise and complete high-resolution structure to date of the S. aureus 70S ribosome with functional ligands.
Identifiants
pubmed: 32852796
doi: 10.1002/1873-3468.13915
doi:
Substances chimiques
Ligands
0
RNA, Messenger
0
RNA, Ribosomal, 16S
0
RNA, Ribosomal, 23S
0
RNA, Transfer
9014-25-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3551-3567Informations de copyright
© 2020 Federation of European Biochemical Societies.
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