The dynamic landscape of transcription initiation in yeast mitochondria.

Adenosine Triphosphate DNA, Fungal / genetics DNA-Directed RNA Polymerases / genetics Fluorescence Resonance Energy Transfer Mitochondria / genetics Mitochondrial Proteins / genetics RNA, Fungal / genetics Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Single Molecule Imaging / methods Transcription Elongation, Genetic Transcription Factors / genetics Transcription Initiation, Genetic

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
27 08 2020
Historique:
received: 16 10 2019
accepted: 14 07 2020
entrez: 29 8 2020
pubmed: 29 8 2020
medline: 25 9 2020
Statut: epublish

Résumé

Controlling efficiency and fidelity in the early stage of mitochondrial DNA transcription is crucial for regulating cellular energy metabolism. Conformational transitions of the transcription initiation complex must be central for such control, but how the conformational dynamics progress throughout transcription initiation remains unknown. Here, we use single-molecule fluorescence resonance energy transfer techniques to examine the conformational dynamics of the transcriptional system of yeast mitochondria with single-base resolution. We show that the yeast mitochondrial transcriptional complex dynamically transitions among closed, open, and scrunched states throughout the initiation stage. Then abruptly at position +8, the dynamic states of initiation make a sharp irreversible transition to an unbent conformation with associated promoter release. Remarkably, stalled initiation complexes remain in dynamic scrunching and unscrunching states without dissociating the RNA transcript, implying the existence of backtracking transitions with possible regulatory roles. The dynamic landscape of transcription initiation suggests a kinetically driven regulation of mitochondrial transcription.

Identifiants

DOI: 10.1038/s41467-020-17793-2 PMID: 32855416 PMC: PMC7452894
pubmed: 32855416
doi: 10.1038/s41467-020-17793-2
pii: 10.1038/s41467-020-17793-2
pmc: PMC7452894
doi:

Substances chimiques

DNA, Fungal 0
MTF1 protein, S cerevisiae 0
Mitochondrial Proteins 0
RNA, Fungal 0
Saccharomyces cerevisiae Proteins 0
Transcription Factors 0
Adenosine Triphosphate 8L70Q75FXE
DNA-Directed RNA Polymerases EC 2.7.7.6
RPO41 protein, S cerevisiae EC 2.7.7.6

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4281

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM118086
Pays : United States

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Auteurs

Byeong-Kwon Sohn (BK)

School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
ORCID: 0000-0002-9266-532X

Urmimala Basu (U)

Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA.
ORCID: 0000-0003-4035-1246

Seung-Won Lee (SW)

School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

Hayoon Cho (H)

School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

Jiayu Shen (J)

Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA.
ORCID: 0000-0001-8447-9378

Aishwarya Deshpande (A)

Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA.
ORCID: 0000-0001-7278-6047

Laura C Johnson (LC)

Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA.

Kalyan Das (K)

Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, 3000, Leuven, Belgium.
ORCID: 0000-0002-8897-324X

Smita S Patel (SS)

Department of Biochemistry and Molecular Biology, Rutgers University, Robert Wood Johnson Medical School, Piscataway, NJ, 08854, USA. patelss@rwjms.rutgers.edu.
ORCID: 0000-0002-2523-4933

Hajin Kim (H)

School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea. hajinkim@unist.ac.kr.
Institute for Basic Science, Ulsan, Republic of Korea. hajinkim@unist.ac.kr.
ORCID: 0000-0001-9311-0484

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › Adénosine triphosphate › The dynamic landscape of transcription...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN fongique › The dynamic landscape of transcription...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › The dynamic landscape of transcription...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › The dynamic landscape of transcription...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › The dynamic landscape of transcription...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › The dynamic landscape of transcription...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN fongique › The dynamic landscape of transcription...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › The dynamic landscape of transcription...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › The dynamic landscape of transcription...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Imagerie moléculaire › Imagerie de molécules uniques › The dynamic landscape of transcription...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › Élongation de la transcription › The dynamic landscape of transcription...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › The dynamic landscape of transcription...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Transcription génétique › Initiation de la transcription › The dynamic landscape of transcription...